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p3xFLAG CMV14 / BRAF-V600K

Plasmid

#131720

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

V600K

Promoter

CMV

Available Since

Dec. 17, 2022

Availability

Academic Institutions and Nonprofits only

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GFP SRBC

Plasmid

#68399

Purpose

Expression of fluorescently tagged SRBC in mammalian cells

Depositor

Rob Parton

Article

Bastiani et al J Cell Biol. 2009 Jun 29;185(7):1259-73.

Insert

SRBC (Cavin3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pTagGFP2-N SIN1 delta2-29

Plasmid

#124923

Purpose

For expression of a.a.2-29 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23 T230A 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86023

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain: Changed threonine 230 to…

Promoter

CMV IE

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4.23 D637G 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86024

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain: Changed aspartic acid 63…

Promoter

CMV IE

Available Since

April 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-AR-R405S

Plasmid

#126051

Purpose

Expresses human androgen receptor (R405S) in mammalian cells

Depositor

Elizabeth Wilson

Article

Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

Insert

AR (AR Human)

Expression

Mammalian

Mutation

R405S

Promoter

CMV

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 M C-V5-tag

Plasmid

#179986

Purpose

Mammalian expression vector for RaTG13 M, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 M

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594E

Plasmid

#131714

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594E

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-Drosha (NLS-P426)

Plasmid

#171538

Purpose

Encodes mutant human Drosha protein

Depositor

Yan Zeng

Article

Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

Insert

DROSHA (DROSHA Human)

Expression

Mammalian

Mutation

an SV40 NLS sequence (PKKKRKVGI) insertion into t…

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Cherry-SRBC

Plasmid

#69492

Purpose

Expression of fluorescently tagged SRBC in mammalian cells

Depositor

Rob Parton

Article

Gambin et al Elife. 2013 Jan 1;3:e01434. doi: 10.7554

Insert

SRBC (Cavin3 Mouse)

Tags

Cherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-NSD3-short del(1-100)

Plasmid

#72547

Purpose

expresses 3*FLAG tagged human NSD3-short with 1-100 aa deletion

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

1-100 aa deletion

Promoter

LTR

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-G466V

Plasmid

#131722

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

G466V

Promoter

CMV

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23 A82V/T230A 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86025

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain; Changed alanine 82 to va…

Promoter

CMV IE

Available Since

July 20, 2019

Availability

Academic Institutions and Nonprofits only

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Desmoplakin II no force control (FL-based)

Plasmid

#118724

Purpose

The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

Depositor

Carsten Grashoff

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)

Use

Retroviral

Tags

YPet(short)-FL-mCherry

Expression

Mammalian

Mutation

truncation after aa1353

Promoter

CMV

Available Since

Dec. 27, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + M-AAT target

Plasmid

#86008

Purpose

lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
M-AAT target

Use

Lentiviral

Mutation

V30M

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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FASTK gRNA (BRDN0001144738)

Plasmid

#77876

Purpose

3rd generation lentiviral gRNA plasmid targeting human FASTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FASTK (FASTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FASTK gRNA (BRDN0001145485)

Plasmid

#77877

Purpose

3rd generation lentiviral gRNA plasmid targeting human FASTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FASTK (FASTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KHBD00054

Plasmid

#34216

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG2275 (Jra Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
GfaABC1D-flag-mAdrb1-WPRE

Plasmid

#223671

Purpose

AAV expression of flag-mAdrb1 from GfaABC1D promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mAdrb1 (Adrb1 Mouse)

Use

AAV

Tags

FLAG

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-flag-mAdrb1-mNeonGreen-WPRE

Plasmid

#223672

Purpose

AAV expression of flag-mAdrb1-mNeonGreen from GfaABC1D promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mAdrb1-mNeonGreen (Adrb1 Mouse)

Use

AAV

Tags

FLAG and mNeonGreen

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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