We narrowed to 5,482 results for: pcas
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pX330 NC-FKBP-Cas9
Plasmid#162725PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459_HR_Prnp_3
Plasmid#78623PurposepX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequenceDepositorInsertHR_Prnp_3 (Prnp Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
myc-hRAD18 dC2
Plasmid#68831PurposeExpresses c-myc-tagged human RAD18 deleting Polymerase eta binding domainDepositorInsertc-myc tagged human RAD18 deleting Polymerase eta binding domain (RAD18 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
FL MALT1 Catalytic Mutant in pET29b-Strep
Plasmid#48969PurposeExpresses Full length catalytic mutant MALT1 in e.coliDepositorInsertMALT1 isoform A (MALT1 Human)
UseTagsHis and StrepExpressionBacterialMutationCatalytic Mutant C464APromoterT7Available sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
myc-hRAD18 d6BD
Plasmid#68832PurposeExpresses c-myc-tagged human RAD18 deleting hRAD6 binding domainDepositorInsertc-myc tagged human RAD18 deleting hRAD6 binding domain (RAD18 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiGuideFB-Puro-A
Plasmid#192506PurposeFor cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.DepositorInserthU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiGuideFB-Puro-B
Plasmid#192507PurposeFor cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.DepositorInsertbU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE
UseLentiviralTagsExpressionMutationPromoterbU6Available sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-RORb2-IRES-GFPd2
Plasmid#73998PurposePlasmid expressing RORb2-IRES-GFPd2DepositorInsertRORb2 ORF (Rorb Mouse)
UseTagsnoneExpressionMammalianMutationPromoterCAGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_HPRT1_exon3_2_Lb
Plasmid#155054PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of HPRT1 exon 3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330 L-FKBP-Cas9
Plasmid#162728PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_HPRT1_exon2_1_Lb
Plasmid#155051PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of HPRT1 exon 2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT131_GalL_cCDA1-NGBE3
Plasmid#145095PurposeExpressing base editor cCDA1-NGBE3 in yeast cellsDepositorInsertcCDA1-NGBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21 Catalytic Domain of MALT1 Catalytic Mutant
Plasmid#48971PurposeExpresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566DepositorInsertMALT1 (MALT1 Human)
UseTagsHisExpressionBacterialMutationContains AA 329-566; Catalytic Mutant C464APromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA1
Plasmid#113130PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6 for gRNA expression, RSV for GFP expressionAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
SIN-CMV-Ca9-WPRE
Plasmid#87874PurposeTransfer plasmid for the production of lentiviral vectorsDepositorInsertspCas9
UseLentiviralTagsnlsExpressionMutationHuman codon-optimizedPromoterCMVAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dicer_1
Plasmid#68807Purposespecific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.DepositorInsertsgRNA mouse Dicer1
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCJ1023
Plasmid#228763PurposePlasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.DepositorUseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterhuman U6Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-TP53
Plasmid#227318PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of TP53 for knock-in.DepositorInsertsgRNA Targeting N-terminus of TP53 (TP53 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-MAPRE1
Plasmid#207793PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.DepositorInsertsgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only
