We narrowed to 5,561 results for: Chia

1. pJL NarX Myc

   Plasmid

   #225651

   Purpose

   Expresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   NarX (narX E. coli)
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPICZ A-Strep-Opa1 (SB251)

   Plasmid

   #227600

   Purpose

   Inducible expression of Strep-tagged Opa1 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   OPA1 (OPA1 Human)
   

   Use

   Tags

   Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 253-960 only

   Promoter

   AOX1

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWR88

   Plasmid

   #203422

   Purpose

   empty BLINCAR plasmid for payload delivery, no payload, no genomic target

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   CAR copy 1
   P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
   CAR copy 2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXU01

   Plasmid

   #212151

   Purpose

   Plasmid expressing xylitol dehydrogenase under inducible promoter PT7 control. Plasmid confers the ability to use xylitol as major carbon source to E. coli.

   Depositor

   Kang Zhou

   Article

   Fung et al Biomaterials. 2022 Aug;287:121661. doi:

   Insert

   xylitol dehydrogenase
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   PT7

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ABE-RA5.5

   Plasmid

   #208316

   Purpose

   Expresses ABE-RA5.5 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA5.5-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-PGUS

   Plasmid

   #207827

   Purpose

   FLAG-tagged PGUS driven by CMV promoter

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   PGUS
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3

   Plasmid

   #201534

   Purpose

   Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Dan Park

   Article

   Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

   Insert

   ilvA-relE_RBS3_CB3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   rhaS W190G

   Promoter

   PrhaBAD (rhamnose)

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL0_CD-GUS

   Plasmid

   #202137

   Purpose

   Level 0 plasmid containing a beta glucuronidase reporter gene with CD overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   Beta glucuronidase gene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pOET3-C-TEV-Twin-Strep

   Plasmid

   #203324

   Purpose

   Generate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   TEV cleavable site (ENLYFQG) and Twin-strep-tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBEL1954

    Plasmid

    #195689

    Purpose

    Expression/purification of 6xHis-TEV-MlaD(F118A/L123A)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaD(F118A/L123A)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBEL1936

    Plasmid

    #195692

    Purpose

    Complementation of MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBEL1935

    Plasmid

    #195693

    Purpose

    Complementation of MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEL1953

    Plasmid

    #195694

    Purpose

    Complementation of MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEL1933

    Plasmid

    #195695

    Purpose

    Complementation of MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBEL1934

    Plasmid

    #195696

    Purpose

    Complementation of MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBEL2707

    Plasmid

    #195636

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBEL2753

    Plasmid

    #195637

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I241N)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251; I241N)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEL2754

    Plasmid

    #195638

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251; L245N)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBEL2755

    Plasmid

    #195639

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251; I248N)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEL2756

    Plasmid

    #195640

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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