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CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

Plasmid

#225756

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIN-TetR-mCherry

Plasmid

#59417

Purpose

retroviral construct expressing the Tet repressor (TetR) tagged with mCherry

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repressor protein (TetR)

Use

Retroviral

Tags

mCherry

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pSLC-284

Plasmid

#124294

Purpose

A low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.

Depositor

Swaine Chen

Article

Eshaghi et al Pathogens. 2016 Jan 5;5(1). pii: pathoge

Insert

vsfGFP-9

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pNSA-FLUC

Plasmid

#98143

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, zeocin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTGFP-T7-Hha10T

Plasmid

#65427

Purpose

Parent vector for constructing non-replicating lesion-bearing plasmid

Depositor

Yinsheng Wang

Article

You et al Nat Protoc. 2015 Sep;10(9):1389-406. doi

Type

Empty backbone

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pHR'CMVGFP_hRIF1(1924-2446)IREShygro

Plasmid

#23138

Depositor

Lifeng Xu

Article

Xu et al J Cell Biol. 2004 Dec 6. 167(5):819-30.

Insert

RIF1 deletion construct (RIF1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 17, 2010

Availability

Academic Institutions and Nonprofits only

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pRSETb-Ferritin-Dronpa

Plasmid

#101194

Purpose

Expresses a Ferritin-Dronpa fusion construct in E. coli

Depositor

Peter Dedecker

Article

Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

Insert

Ferritin-Dronpa

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

Plasmid

#225755

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-HPH-C4

Plasmid

#160343

Purpose

High-copy expression vector for K. marxianus, hygromycin selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBPGUw-HACK-G4>QF2

Plasmid

#80277

Purpose

GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Expression

Insect

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-G418-C4

Plasmid

#160342

Purpose

High-copy expression vector for K. marxianus, G418 selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pXT130_TwinStrep-SUMO-ShTnsC

Plasmid

#135526

Purpose

Protein expression construct for ShTnsC

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsC

Tags

TwinStrep-SUMO

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-URA-C4

Plasmid

#160339

Purpose

High-copy expression vector for K. marxianus, uracil selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBHM2679

Plasmid

#241724

Purpose

Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSD marker for selection on blasticidin S

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-URA

Plasmid

#160004

Purpose

Low-copy expression vector for K. marxianus, uracil selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

n/a

Expression

Yeast

Promoter

n/a

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-TcRb:mCherry-FKBP

Plasmid

#72904

Purpose

ER-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

T cell receptor β chain

Use

Lentiviral

Tags

biotinylation acceptor peptide (AP) and mCherry, …

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pXT131_TwinStrep-SUMO-ShTniQ

Plasmid

#135527

Purpose

Protein expression construct for ShTniQ

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTniQ

Tags

TwinStrep-SUMO

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pXT129_TwinStrep-SUMO-ShTnsB

Plasmid

#135525

Purpose

Protein expression construct for ShTnsB

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB

Tags

TwinStrep-SUMO

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2_claudink:Cas9green; control_gRNA

Plasmid

#199336

Purpose

pDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

claudink
Cas9
control sgRNA

Use

Tol2 destination vector

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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SCP1-CMVenh-luc

Plasmid

#22216

Depositor

James Kadonaga

Article

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP1 (super core promoter 1)-CMVenh luciferase

Use

Luciferase

Expression

Mammalian

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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