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CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)

Plasmid

#117856

Purpose

This plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.

Depositor

C. Ron Yu

Article

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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Dscaml1-Fc-His

Plasmid

#72072

Purpose

Expresses the extracellular region of the Dscaml1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscaml1 (Dscaml1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-actin-pcDNA4-TO

Plasmid

#118370

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Tags

HA-GFP1-10

Expression

Mammalian

Promoter

CMV/TO

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc FL

Plasmid

#221853

Purpose

Express human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

calreticulin (CALR Human)

Tags

Human IgG1Fc tag

Expression

Mammalian

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)

Plasmid

#99343

Purpose

Bacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TAD

Depositor

Peter Wright

Article

Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

Inserts

Hif1a (776-826) (HIF1A Human)
CBP (340-439)

Tags

His6GB1

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTarget_AT1R-tTA

Plasmid

#222851

Purpose

Mammalian expression plasmid encoding the human Angiotensin II type I receptor fused to the tTA transcriptional activator

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT1R (AGTR1 Human)

Tags

FLAG and tTA

Expression

Mammalian

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ALDH1A1

Plasmid

#189749

Purpose

The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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-53 bp human IFN-gamma luc

Plasmid

#17597

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-53 to +70 of promoter

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
SITS-MmCav1-EGFP-APEX2

Plasmid

#170525

Purpose

Vector expression of MmCav1-EGFP-APEX2 in Leishmania cell free system. Parton lab clone JEJ

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Synthetic Biology

Tags

APEX2

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-Rosmarinus

Plasmid

#183462

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

Rosmarinus

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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human a5 full length

Plasmid

#217818

Purpose

Full length human integrin a5 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGA5 (ITGA5 Human)

Tags

P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ashL344P-mCerulean

Plasmid

#69581

Purpose

Expresses p53-L344P mutant tagged with mCerulean

Depositor

Galit Lahav

Article

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53-L344P (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

p53 L344P mutation that prevents oliogomerization…

Promoter

EF1alpha

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-AID'-seBE4max-IRES

Plasmid

#174697

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

AID'-seBE4max-IRES (AICDA Human)

Tags

myc tag

Expression

Mammalian

Mutation

UGI-E11K

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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ADRA2A-DuET

Plasmid

#213177

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

ADRA2A (ADRA2A Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRC55-GFP-DHX37

Plasmid

#192150

Purpose

express GFP-DHX37

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Insert

GFP-DHX37 (Dhx37 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mCarmine

Plasmid

#183464

Purpose

To produce AAV with the P3 promoter to express Alb-mCarmine fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mCarmine

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pVAX1-BMP2/7 -

Plasmid

#137912

Purpose

Constitutive mammalian co-expression vector for human bone morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, divergent)

Depositor

Georg Feichtinger

Article

Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

Inserts

BMP2 (BMP2 Human)
BMP7 (BMP7 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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phBG-P53DD

Plasmid

#149707

Purpose

In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

Depositor

Erika Sasaki

Article

Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

Insert

Trp53 (Trp53 Mouse)

Use

Rna transcription

Mutation

deleted amino acid 14-301

Promoter

T7

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBSWHhc-p53

Plasmid

#133903

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (TP53 Mouse)

Expression

Yeast

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-plk1-AKAP

Plasmid

#132967

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1-AKAP (PLK1, AKAP9 Human)

Expression

Mammalian

Mutation

C terminus of full-length Plk1 fused to the peric…

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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