We narrowed to 11,757 results for: pcDNA

1. pcDNA5/FRT/TO_eGFP-pScaC

   Plasmid

   #228824

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaC
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   aa 33-223

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5/FRT/TO_eGFP-pScaA

   Plasmid

   #228823

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaA in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaA
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   aa 25-1216

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA 3.1-NbON10-NanoLuc

   Plasmid

   #198691

   Purpose

   Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture

   Depositor

   Gualberto González Sapienza

   Article

   Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

   Insert

   anti-SARS-CoV-2 Nucleocapsid nanobody ON10
   

   Use

   Tags

   6xHis tag, IgK leader sequence, NanoLuc, and twin…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-AntiHer2-LC122-TAG

   Plasmid

   #226839

   Purpose

   Contains trastuzumab with a TAG codon at position 122 of the light chain for ncAA incorporation

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

   Inserts

   anti-HER2 light chain
   anti-HER2 heavy chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   amino acid 122 of light chain mutated to TAG

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-AntiHer2-HC121-TAG

   Plasmid

   #226836

   Purpose

   Contains trastuzumab with a TAG codon at position 121 of the heavy chain for ncAA incorporation

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

   Inserts

   anti-HER2 light chain
   anti-HER2 heavy chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   amino acid 121 of heavy chain mutated to TAG

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-AntiHer2-HC197-TAG

   Plasmid

   #226838

   Purpose

   Contains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporation

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

   Inserts

   anti-HER2 light chain
   anti-HER2 heavy chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   amino acid 197 of heavy chain mutated to TAG

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5-FRT-TOgw-C-mEGFP

   Plasmid

   #223953

   Purpose

   Gateway cloning of genes of interest with a C-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Roko Žaja, Karla Feijs

   Type

   Empty backbone

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5-FRT-TOgw-C-mECFP

   Plasmid

   #223950

   Purpose

   Gateway cloning of genes of interest with a C-terminal mECFP fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Roko Žaja, Karla Feijs

   Type

   Empty backbone

   Use

   Tags

   mECFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-FRT-TOgw-C-mEYFP

   Plasmid

   #223951

   Purpose

   Gateway cloning of genes of interest with a C-terminal mEYFP fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Roko Žaja, Karla Feijs

   Type

   Empty backbone

   Use

   Tags

   mEYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-FRT-TOgw-N-mCherry

    Plasmid

    #223948

    Purpose

    Gateway cloning of genes of interest with an N-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Roko Žaja, Karla Feijs

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT-TOgw-N-mEGFP

    Plasmid

    #223949

    Purpose

    Gateway cloning of genes of interest with an N-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Roko Žaja, Karla Feijs

    Type

    Empty backbone

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)

    Plasmid

    #187727

    Purpose

    Express his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L with residues 226-382 removed (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    deletion of residues 226-382

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)

    Plasmid

    #187728

    Purpose

    Express his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L with residues 418-495 removed (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    deletion of residues 418-495

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-myc-GFP-TNRC6A-SD-WT

    Plasmid

    #215897

    Purpose

    Expression of TNRC6A-silencing domain (SD)

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3puro-mGFP-FXR1a-Ccswap

    Plasmid

    #225460

    Purpose

    Express EGFP-fusion protein of FXR1 isoform a (CCswap)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CCswap (swapped positions of CC2 and CC1)

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3puro-EGFP-GAPDH-R rich

    Plasmid

    #225480

    Purpose

    Express mEGFP-GAPDH-Rrich fusion

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    GAPDH fused with R-rich region of TDRD3 (GAPDH Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3puro-mGFP-FXR1a-I304N

    Plasmid

    #225461

    Purpose

    Express mEGFP-fusion protein of FXR1 isoform a (I304N)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    I304N

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3puro-EGFP-GAPDH-Tudor

    Plasmid

    #225479

    Purpose

    Express mEGFP-GAPDH-Tudor fusion

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    GAPDH fused with Tudor domain of TDRD3 (GAPDH Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3puro-EGFP-GAPDH-RGG

    Plasmid

    #225477

    Purpose

    Express mEGFP-GAPDH-RGG fusion

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    GAPDH fused with RGG domain of TOP3B (GAPDH Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-Myc-BirA*-CAMK2D

    Plasmid

    #221686

    Purpose

    Mammalian expression of Myc-tagged BirA*-CAMK2D fusion protein for BioID

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge