We narrowed to 9,194 results for: Pol;

1. pCellFree_G03 FYN

   Plasmid

   #67106

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   FYN (FYN Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. p.UTA.2.0-GL-2

   Plasmid

   #82440

   Purpose

   Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2

   Depositor

   Jens Gruber

   Article

   Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

   Insert

   Luciferase
   

   Mutation

   The complementary region for GL2 siRNA control wa…

   Available Since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. gRD21A-mRFP

   Plasmid

   #181959

   Purpose

   RD21A protein tagged with mRFP, under control of native promoter

   Depositor

   George Haughn

   Article

   Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

   Insert

   RD21A genomic sequence including 2kb upstream of the ATG
   

   Tags

   mRFP

   Expression

   Plant

   Promoter

   RD21A native

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FLAG-EAF2 pBacPAK8

   Plasmid

   #15326

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

   Insert

   ELL Associated Factor 2 (EAF2 Human)
   

   Use

   Baculovirus construction

   Tags

   FLAG

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pLyGo-Kp-2

   Plasmid

   #163144

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIS1 DICER1 short UTR

   Plasmid

   #21648

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Dicer 1 (DICER1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pWL502-PAM

   Plasmid

   #174382

   Purpose

   Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   PAM-spacer
   

   Use

   CRISPR; Archaeal expression

   Expression

   Bacterial

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBW-m4046938

   Plasmid

   #145599

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCellFree_G03 CREBZF

   Plasmid

   #67070

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CREBZF (CREBZF Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. MET FLAG103QDProCFPp303

    Plasmid

    #15591

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Tags

    CFP and FLAG

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pGEM-myr-tdTomato-SV40pA-FKF

    Plasmid

    #119867

    Purpose

    Donor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    2x myristoylation

    Expression

    Bacterial

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p21 promoter D

    Plasmid

    #16465

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pCRW0.8 p21 (rat)

    Plasmid

    #16477

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 (Cdkn1a Rat)
    

    Expression

    Bacterial

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pIS1 Cyclin D2 long-mut-miR15/16

    Plasmid

    #21647

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Cyclin D2 long-mut-miR15/16 (CCND2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant miR15/16 microRNA binding sites

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. psicheck-2-Mut mod

    Plasmid

    #48165

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of Mut (Mut Mouse)
    

    Use

    Luciferase

    Mutation

    A/T mutation in the ATG of the uORF

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pBP-ORF

    Plasmid

    #72949

    Purpose

    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 0 - ORFs
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCellFree_G03 CSNK2A1

    Plasmid

    #67085

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pMpGWB106-OEP7(1–50)

    Plasmid

    #100602

    Purpose

    Binary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plants

    Depositor

    Yutaka Kodama

    Article

    Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

    Insert

    OEP7(1–50)-Citrine (OEP7 Mustard Weed)
    

    Expression

    Plant

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p21 promoter F

    Plasmid

    #16467

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

20. GAL 25Q+ProCFPp303

    Plasmid

    #15574

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt25Q + Pro (HTT Human)
    

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only