We narrowed to 46,665 results for: cha;

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NsvBa

Plasmid

#100015

Purpose

Electrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFP

Depositor

David Clapham, Paul DeCaen

Article

DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

Insert

NsvBa

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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mOtop3_pcDNA3

Plasmid

#114679

Purpose

This construct was used to express mouse Otop3 in HEK-293 cells

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 3 (Otop3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-optoB2-SS-YFP

Plasmid

#75270

Purpose

Arrestin independent version of optoB2 with serine mutations at S362A,S363G

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

Arrestin independent version of optoB2

Tags

eGFP

Expression

Mammalian

Mutation

serine mutations at S362A,S363G

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-mEPLIN beta

Plasmid

#40956

Depositor

Elizabeth Luna, Scott Maul

Article

Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

Insert

EPLIN beta (Lima1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pBAD-TRP120

Plasmid

#71732

Purpose

inducible expression of TRP120 in E. coli

Depositor

Jere McBride

Article

Luo et al Clin Vaccine Immunol. 2009 Jul;16(7):982

Insert

TRP120

Tags

6xHIS and Thioredoxin

Expression

Bacterial

Promoter

pBAD

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
MXS_PGK::rtTA3-bGHpA

Plasmid

#62446

Purpose

MXS_chaining vector with PGK::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHL 1903

Plasmid

#53020

Purpose

AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Insert

GFP

Use

Synthetic Biology

Tags

AAV, Asp terminator, and fimS

Expression

Bacterial

Promoter

fimS

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
TetO-FUW-mWnt1

Plasmid

#176486

Purpose

mouse Wnt1 cDNA in TetO backbone

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

mouse Wnt1 cDNA (Wnt1 Mouse)

Use

Lentiviral

Promoter

TetO

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::HygroR-bGHpA

Plasmid

#62436

Purpose

MXS_chaining vector with PGK::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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EZ-L439

Plasmid

#169146

Purpose

Encodes OptoINVRT7 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2020 Dec 18;9(12):3254-3

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSBL_DK187

Plasmid

#156423

Purpose

Insect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmid

Depositor

Stephen Brohawn

Article

Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

Insert

SARS-CoV-2 3a protein

Tags

sfGFP-His

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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POM42-mCherry

Plasmid

#69856

Purpose

N-terminal tagging with mCherry using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCherry minus start and stop codon

Expression

Yeast

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJM126

Plasmid

#49339

Purpose

bacterial expression of RFA1, RFA2, and RFA3

Depositor

Steven Brill

Article

He et al J Biol Chem. 1996 Nov 8;271(45):28243-9.

Inserts

RFA3 (RFA3 Budding Yeast)
RFA2 (RFA2 Budding Yeast)
RFA1 (RFA1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only
pCfB9336 (pgRNA_II-1_NatMX)

Plasmid

#161589

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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L1_KanR-Ck1

Plasmid

#136123

Purpose

L1 in position 1 (next to LB in L2 construct), for KanR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpEF1a:nptII_3t35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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rtTA and TagBFP cassettes

Plasmid

#62452

Purpose

MXS_chaining vector with 2 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::NLS-TagBFPx3-bGHpA
CMV::rtTA3-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::HygroR-bGHpA

Plasmid

#62435

Purpose

MXS_chaining vector with CMV::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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UMG-SP-1_pET29b

Plasmid

#215433

Purpose

Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-1

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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UMG-SP-2_pET29b

Plasmid

#215434

Purpose

Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-2

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRG710_lexO-HO_LexA-TF_HIS3MX

Plasmid

#154819

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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