We narrowed to 10,632 results for: t7
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knob-s48i48
Plasmid#68391PurposeKnob protein fused to elastin-like polypeptide (S48I48)DepositorInsertKnob-S48I48
TagsKnobExpressionBacterialPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETx-SR6v15.t
Plasmid#176214PurposeBacterial expression of the SR6v15.t targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6v15.t
Tags6XHisExpressionBacterialPromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-pchA WT
Plasmid#87102Purposeexpresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosaDepositorInsertpchA WT
Tagshis-tagExpressionBacterialPromoterT7Available SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTXG-hTDG-K330A
Plasmid#52282PurposeBacterial expression of human TDG-K330A C-terminally fused to GyrA intein and GSTDepositorInserthTDG (TDG Human)
TagsGyrA-intein-GSTExpressionBacterialMutationK330A, SUMO acceptor site mutationPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vta1(280-330)
Plasmid#87738PurposeBacterial expression of the VSL domain of S. cerevisiae Vta1DepositorInsertVacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
Tags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-279PromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET29NK-LGK/mGFPmut3
Plasmid#81168PurposeGFP fusion to levoglucosan kinaseDepositorInsertLevoglucosan kinase wild-type
TagsHis6x and mGFPmut3ExpressionBacterialPromoterT7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_H303A
Plasmid#67730PurposeExpresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged histidine 303 to alaninePromoterT7Available SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_D230A
Plasmid#67725PurposeExpresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged aspartate 230 to alaninePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_N191A
Plasmid#67724PurposeExpresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged asparagine 191 to alaninePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_K295A
Plasmid#67726PurposeExpresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged lysine 295 to alaninePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDiySpike2
Plasmid#136949PurposePlasmid for the In Vitro transcription of DiySpike2DepositorInsertDiySpike2
UseSynthetic BiologyPromoterT7Available SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-stl
Plasmid#51721Purposeconditional gene knockdowns in Trypanosoma bruceiDepositorTypeEmpty backboneUseConditional gene knockdown in t. bruceiPromoterT7 (2X)Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2H2D
Plasmid#67081PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDM_noro_A1_lacZA
Plasmid#118818PurposeT7 RNAP-driven expression of norovirus antisense orientation toehold switches (A1) with a lacZ alpha subunit reporter.DepositorInsertNorovirus toehold switch A1
ExpressionBacterialAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZF-ADD-GCN
Plasmid#127475Purposecoding sequence for synthetic zinc finger ZF-ADD-GCNDepositorInsertZF-ADD synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperPromoterT7Available SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
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