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pBTK229

Plasmid

#110591

Purpose

BTK Type 3 coding sequence for golden gate assembly and homologous recombination

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Kanamycin resistance

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEY139

Plasmid

#223486

Purpose

tph-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tph-1 (tph-1 Nematode)

Expression

Worm

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBTK520

Plasmid

#110604

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLib6.4-Aalb_743

Plasmid

#176662

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-B4E

Plasmid

#162067

Purpose

For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E

Depositor

Karen Polizzi

Article

Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1

Insert

ampR-eIF4E fusion protein

Tags

6xHis tag

Expression

Bacterial

Mutation

eIF4E incorporating the K119A mutation, which is …

Promoter

T7

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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BB1_12_pGAP

Plasmid

#98498

Purpose

BB1 with insert: promoter PGAP

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

GAP promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK510

Plasmid

#110611

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 30, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK599

Plasmid

#110600

Purpose

BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP dropout

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK564

Plasmid

#110613

Purpose

BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Nanoluc

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLib6.4-Consensus

Plasmid

#176664

Purpose

Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aaeg_774

Plasmid

#176659

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8 dCD

Plasmid

#121117

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 72-389 (CBX8 Human)

Use

Lentiviral

Tags

V5

Mutation

contains amino acids 77-389

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8 3xRA

Plasmid

#121115

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 (R19A, R20A, R22A) (CBX8 Human)

Use

Lentiviral

Tags

V5

Mutation

R19A, R20A, R22A

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK615

Plasmid

#110609

Purpose

BTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

sgRNA

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK509

Plasmid

#110610

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pILGFP95

Plasmid

#83550

Purpose

Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

PDA1 promoter-yEGFP

Expression

Yeast

Promoter

PDA1

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only
pBTK121

Plasmid

#110581

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

PA3 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pILGFPH7

Plasmid

#83554

Purpose

Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

ADH2 promoter-yEGFP

Expression

Yeast

Promoter

ADH2

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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CaM12(D20A/D56A)/pIRES2-eGFP

Plasmid

#111512

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Expression

Mammalian

Mutation

D20A/D56A

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a(TIGIT)

Plasmid

#177902

Purpose

Bacterial expression of his10-tagged human TIGIT Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

TIGIT (aa 163-244 only) (TIGIT Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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