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Plasmid#231081PurposeExpresses PLAG1 with C-terminal 3xFLAG tag, in doxycycline-inducible mannerDepositorInsertPLAG1 (PLAG1 Human)
UseLentiviralTags3XFLAGExpressionMutationPromoterAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCLAP
Plasmid#200786PurposeCloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.DepositorTypeEmpty backboneUseTagsCLIPf and SNAPf-6xHis-FLAGExpressionMammalianMutationPromoterAvailable sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
mEosEM-E_N1
Plasmid#226337PurposePhotoswitchable fluorescent protein for correlative light-electron microscopy; empty backbone containing mEosEM-E for mammalian expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA
Plasmid#222861PurposeThis plasmid codes for the OgeuIscB nickaseDepositorInsertOgeuIscB Nickase
UseCRISPRTags3X HA and Nucleoplasmin NLS and ITR2ExpressionMammalianMutationE193A for nickase mutationPromoterCMVAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiGuideB3GNT5-neo
Plasmid#220867Purposeencodes CRISPR sgRNA targeting human B3GNT5DepositorInsertB3GNT5 (B3GNT5 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT172
Plasmid#218734PurposeExpresses N-terminally EGFP-tagged IFT172 in mammalian cellsDepositorInsertIFT172 (IFT172 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmax-PUFc_NES-ADARdd(E488Q)
Plasmid#196837PurposeExpress PUFc_NES-ADARdd(E488Q)DepositorInsertPUFc_NES-ADARdd(E488Q)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmax-MCP_NES-ADARdd(E488Q)
Plasmid#196840PurposeExpress MCP_NES-ADARdd(E488Q)DepositorInsertMCP_NES-ADARdd(E488Q)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hCAR.Variant3
Plasmid#209133PurposeThe ORF of human constitutive androstane receptor (CAR), variant 3 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.DepositorInsertconstitutive androstane receptor (CAR), Variant 3 (NR1I3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
vYM004_pNeo_phiC31_nTRE_pEF1s(orig)_citrine
Plasmid#197677Purpose"pEF1s(orig)" reporter plasmidDepositorInsertpEF1s-H2B-Citrine
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-BclXL-ActA-pEGFP-C1
Plasmid#177412PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).DepositorInsertBcl-XL-ActA, BclX-acta
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl2-ActA-pEGFP-C1
Plasmid#177418PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).DepositorInsertBcl-2-ActA, Bcl2-acta, Bcl-acta
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl2-cb5-pEGFP-C1
Plasmid#177421PurposeExpress mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).DepositorInsertBcl-2-Cb5, Bcl2-cb5, Bcl-cb5
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-RFP_mmu-miR-292b-3p_sponge
Plasmid#132623PurposeExpresses a TurboRFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA spongesDepositorInsertstop codon, mmu-miR-292b-3p sponge sequence and polyA signal
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-79aa-ActA-pEGFP-C1
Plasmid#166763PurposeTo target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.DepositorInsertActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO-neo NXPH4-sh3
Plasmid#163746PurposeLentivirus for inducible knockdown of NXPH4 (human)DepositorInsertNXPH4 (NXPH4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterTREAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO-neo NXPH4-sh2
Plasmid#163745PurposeLentivirus for inducible knockdown of NXPH4 (human)DepositorInsertNXPH4 (NXPH4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterTREAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO-neo NXPH4-sh1
Plasmid#163744PurposeLentivirus for inducible knockdown of NXPH4 (human)DepositorInsertNXPH4 (NXPH4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterTREAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pStrep-5xEGFP-N1
Plasmid#122492PurposeExpression of Strep-tagged 5xEGFPDepositorInsertStrep-tagged 5xEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStrep-7xEGFP-N1
Plasmid#122494PurposeExpression of Strep-tagged 7xEGFPDepositorInsertStrep-tagged 7xEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only