We narrowed to 7,402 results for: Lif;

1. pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

   Plasmid

   #205098

   Purpose

   Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. d

   Insert

   mNeonGreen, eKL9h, and Volvox channelrhodopsin-1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. P119 C-Check

   Plasmid

   #66817

   Purpose

   Dual-fluorescent surrogate reporter vector for in vitro functional assay of programable DNA nuclease and enrichment of genetically edited cells

   Depositor

   Yonglun Luo

   Article

   Zhou et al Cell Mol Life Sci. 2016 Jan 11.

   Insert

   truncated Enhanced Green Fluorescent Protein and asRED
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK and CMV

   Available since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-EGFP-C1-HsHelz_AG

   Plasmid

   #148799

   Purpose

   Mammalian Expression of HsHelz

   Depositor

   Elisa Izaurralde

   Article

   Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

   Insert

   HsHelz (HELZ Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe puro-HA-PIK3CA(H1047R;I848V)

   Plasmid

   #33253

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   changed histidine 1047 to arginine and isoleucine…

   Promoter

   SV40

   Available since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pENTR1A mKate2-hu_ncOGT D554N

   Plasmid

   #154283

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Expression

   Mutation

   D554N mutation (Ser/Thr Glycosylation Impaired)

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. anxA10-GFP

   Plasmid

   #107199

   Purpose

   Expresses human annexin A10-GFP fusion protein for live cell imaging

   Depositor

   Ursula Rescher, Volker Gerke

   Article

   Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

   Insert

   annexin A10 (ANXA10 Human)
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICSL11055

   Plasmid

   #68252

   Purpose

   Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-Puro-TDP-43-D169G

   Plasmid

   #133757

   Purpose

   Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed aspartate 169 to glycine.

   Promoter

   CMV

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBpuro-ErbB1(DEL1011)

   Plasmid

   #197366

   Purpose

   Encoding human ErbB1 mutant.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   EGFR mutant. (EGFR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted amino acids 1011-1186

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pScalps_Puro_mCebpe

    Plasmid

    #79076

    Purpose

    Expression of mouse Cebpe

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Cebp epsilon (Cebpe Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLPC-Flag-CHES1L

    Plasmid

    #51836

    Purpose

    encodes the long isoform of human CHES1 and a Flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    CHES1 (FOXN3 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

    Plasmid

    #102986

    Purpose

    AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    membrane-targeted SNAP protein after FLP-mediated recombination
    Nuclear-targeted eGFP after FLP-mediated recombination
    

    Use

    AAV; Adeno associated viral vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. 139H2_LC

    Plasmid

    #206202

    Purpose

    For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.

    Depositor

    Joost Snijder

    Article

    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

    Insert

    anti-MUC1 antibody 139H2 light chain (Igkc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe puro-HA-PIK3CA(H1047R;G320E)

    Plasmid

    #33254

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and glycine 32…

    Promoter

    SV40

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pMSCV-PIG-Lin28b

    Plasmid

    #64795

    Purpose

    Expresses human Lin28b in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Lin28b (LIN28B Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe-HA-Chk2-D311V

    Plasmid

    #41921

    Depositor

    Stephen Elledge

    Article

    Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

    Insert

    Chk2 (CHEK2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D311V

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pBW_0001

    Plasmid

    #102833

    Purpose

    Binary vector containing Sr22 Schomburgk allele driven by maize ubiquitin promoter, Sr22 terminator

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 Schomburgk (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDual-eGFP(H66)

    Plasmid

    #63558

    Purpose

    Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(H66)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed tyrosine at position 66 with respect to t…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pDONR221_SLC7A3

    Plasmid

    #132301

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

    Insert

    SLC7A3 (SLC7A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A

    Plasmid

    #127790

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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