We narrowed to 32,111 results for: eng

1. pRS315-SEC22-UWOPS872421

   Plasmid

   #226272

   Purpose

   Plasmid expressing the SEC22 allele from UWOPS87-2421, under control of its native promoter

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   SEC22 (SEC22 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS315-SEC22-S288C

   Plasmid

   #226270

   Purpose

   Plasmid expressing the SEC22 allele from S288C, under control of its native promoter

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   SEC22 (SEC22 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pML107-PMR1

   Plasmid

   #226264

   Purpose

   Plasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 gene

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   PMR1 gRNA (PMR1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS316-SSD1-NCYC110

   Plasmid

   #226279

   Purpose

   Plasmid expressing the SSD1 allele from NCYC110, under control of its native promoter

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   SSD1 (SSD1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDLterm

   Plasmid

   #211903

   Purpose

   Base plasmid for dual-luciferase constructs to measure terminator strength

   Depositor

   Stan Fields

   Article

   Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Plant

   Promoter

   35S enhancer + 35S minimal promoter

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Zhou-pLOV-GFP-Luc

   Plasmid

   #161030

   Purpose

   Lentiviral vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection.

   Depositor

   Dapeng Zhou

   Article

   Tong et al Vaccines (Basel). 2021 Mar 22;9(3). pii:

   Insert

   GFP and luciferase
   

   Use

   Lentiviral

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJH4506

   Plasmid

   #200255

   Purpose

   Pnpr-4 TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFP

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   TeTx
   

   Tags

   mCherry

   Expression

   Worm

   Promoter

   Pnpr-4

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH4693

   Plasmid

   #200784

   Purpose

   Ptwk-40s EGFP unc-54 3' UTR C.elegans AVA and other neurons expression of EGFP

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   no
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   Ptwk-40s

   Available Since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pJH2673

   Plasmid

   #199691

   Purpose

   Pnmr-1 FLPe unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of FLPe

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   flpE (flpE )
   

   Tags

   no

   Expression

   Worm

   Promoter

   Pnmr-1 long

   Available Since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. TtA_pET29b

    Plasmid

    #215432

    Purpose

    Expression construct for TtA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    TtA
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDonor_RB-TnV2_pJEx

    Plasmid

    #213908

    Purpose

    Barcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sites

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGAL1-VP1 (LEU2)

    Plasmid

    #182480

    Purpose

    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Insert

    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLS.544

    Plasmid

    #184961

    Purpose

    Test effect of a1/a2 length on editing murF using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
    

    Expression

    Bacterial

    Mutation

    murF donor G1359A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNCS-Clover4-LUMABS-HA

    Plasmid

    #183486

    Purpose

    To express the Clover4-LUMABS-HA sensor for Avian Influenza Virus antibody detection.

    Depositor

    Jun Chu, Zongwen Jin

    Article

    Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

    Insert

    Clover4-LUMABS-HA
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ-NF1-L1-dSpRY

    Plasmid

    #177681

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dCas9-SpRY
    

    Use

    CRISPR; Gateway compatible foki-dcas9-spry entry …

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ-NF1-L2-dSpRY

    Plasmid

    #177682

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dCas9-SpRY
    

    Use

    CRISPR; Gateway compatible foki-dcas9-spry entry …

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ-NF-L2-dMb2Cas12a

    Plasmid

    #177684

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dMb2Cas12a
    

    Use

    CRISPR; Gateway compatible foki-dmb2cas12a entry …

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pb mD3S4-dN

    Plasmid

    #180252

    Purpose

    P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.

    Depositor

    Timothy Springer

    Article

    Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:

    Insert

    D3 of P. berghei HAP2 with N-glycosylation sites mutated
    

    Tags

    6His

    Expression

    Mammalian

    Mutation

    N516T, S533N and N539Q

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-4T-1-Rnt1-dsRBD(+6A)

    Plasmid

    #171552

    Purpose

    Encoding part of the budding yeast Rnt1 protein from L366 to S453

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    Rnt1 (RNT1 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    a six-alanine insertion in the L1 region

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T-1-Rnt1-dsRBD(+3A)

    Plasmid

    #171551

    Purpose

    Encoding part of the budding yeast Rnt1 protein from L366 to S453

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    Rnt1 (RNT1 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    a three-alanine insertion in the L1 region

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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