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We narrowed to 14,439 results for: eng

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Showing: 5141 - 5160 of 14439 results

  4. pHybr_r2a>mG1-srt-his

    Plasmid
    #124802
    Purpose
    HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Publication
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Murine IgG1
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Promoter
    -
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124802-plasmid-map-sequence-id-244317

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  8. pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro

    Plasmid
    #234950
    Purpose
    Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Publication
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234950-plasmid-map-sequence-id-467732

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  9. Lenti-DAG1

    Plasmid
    #205149
    Purpose
    Overexpress DAG1
    Depositor
    Monkol Lek
    Insert
    Dystroglycan 1 (DAG1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Carries common missense variant c.41C>G (p.Ser…
    Promoter
    EF-1a
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    205149-plasmid-map-sequence-id-405998

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  10. pcS-E626-C1C2

    Plasmid
    #200162
    Purpose
    This plasmid encodes EGFR targeting monobody (E626) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.
    Depositor
    Masako Harada
    Publication
    Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)
    Inserts
    E626
    C1C2 domain of lactadherin
    Tags
    HA and HIS
    Expression
    Mammalian
    Mutation
    sequence adopted from doi: 10.1371/journal.pone.0…
    Promoter
    CMV
    Available since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200162-plasmid-map-sequence-id-396334

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  11. FRB-GFP-Gab1(Y628F/Y660F)

    Plasmid
    #188658
    Purpose
    FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe
    Depositor
    Andrei Karginov
    Publication
    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GRB associated binding protein 1 (Gab1 Mouse)
    Tags
    FRB and GFP
    Expression
    Mammalian
    Mutation
    Y628F and Y660F
    Promoter
    CMV
    Available since
    Aug. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    188658-plasmid-map-sequence-id-369577

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  13. BE3-hA3A-R128A

    Plasmid
    #132944
    Purpose
    Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Publication
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3-hA3A(R128A)
    Use
    CRISPR; Base editor
    Expression
    Mammalian
    Mutation
    BE3-hA3A(R128A)
    Available since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132944-plasmid-map-sequence-id-314824

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  14. pSCIP-mB2M

    Plasmid
    #154091
    Purpose
    Self-Cutting and Integrating CRISPR backbone targeting murine B2M
    Depositor
    Scott McComb
    Publication
    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA and BAIT targeting murine B2M locus
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    154091-plasmid-map-sequence-id-307093

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  15. pCEP4-DU422gp160

    Plasmid
    #100926
    Purpose
    Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (DU422.1) Env
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100926-plasmid-map-sequence-id-190000

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  16. sgEGFR(A)

    Plasmid
    #85564
    Purpose
    Expresses sgRNA targeting human EGFR around codon T790
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    epidermal growth factor receptor (EGFR Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  20. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

    Plasmid
    #234951
    Purpose
    Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Publication
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1A
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    234951-plasmid-map-sequence-id-467430

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Showing: 5141 - 5160 of 14439 results