We narrowed to 9,203 results for: sacs

1. pDN-T1GZmbh

   Plasmid

   #44522

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
   yEGFP::ZeoR
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Three tandem tet operators (3XtetO2) downstream o…

   Promoter

   PGal1-T123 promoter

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. His6-SMT3-SopB

   Plasmid

   #183676

   Purpose

   Recombinant Salmonella Typhimurium SopB

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   His6-S. cerevisiae SMT3(2-101)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDN-G1TCbt

   Plasmid

   #44515

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
   tetR::ymcherry
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Two tandem tet operators (2XtetO2) downstream of …

   Promoter

   PGal1-D12 promoter

   Available since

   April 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. nuc-yHS1-M7A,H102A

   Plasmid

   #159168

   Purpose

   Nuclear labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   nuc-yHS1-M7A,H102A
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Mutated both Met 7 and His 102 of the cyt b562 mo…

   Promoter

   GPD

   Available since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. mito-yHS1-M7A,H102A

   Plasmid

   #159165

   Purpose

   Mitochondrial labile heme reporter

   Depositor

   Amit Reddi

   Article

   Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

   Insert

   mito-yHS1-M7A,H102A
   

   Use

   Tags

   COX4 pre-sequence

   Expression

   Yeast

   Mutation

   Mutated both Met 7 and His 102 of the cyt b562 mo…

   Promoter

   GPD

   Available since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDONR223-GOLPH3

   Plasmid

   #21688

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Golgi Phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   last nucleotide of stop codon removed for c-termi…

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

   Plasmid

   #165492

   Purpose

   Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
   

   Use

   AAV

   Tags

   darkVenus

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. deltaVP1 + VP2C-GFP

   Plasmid

   #166676

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFP
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDN-G1GZmbh

   Plasmid

   #44516

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
   yEGFP::ZeoR
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Two tandem tet operators (2XtetO2) downstream of …

   Promoter

   PGal1-D12 promoter

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLINK cyclin T1 (1-280) myc (all 200) (P#575)

    Plasmid

    #14610

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-280) (all 200) (CCNT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    C198A, C200A, H202A, C205A. Active in 3T3 cells.

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. AIDx-ID-1058

    Plasmid

    #135347

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-1058-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-ABEMAX-NLS-P2A_BLAST

    Plasmid

    #135355

    Purpose

    Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax-2XNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-6P-1 p37 SHPmut

    Plasmid

    #113503

    Purpose

    Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    F215A/E218A/QKL220-222AAA

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail

    Plasmid

    #60392

    Purpose

    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast, Synthetic)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4), amino a…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pGEX-6P-1 p37 deltaUBX

    Plasmid

    #113504

    Purpose

    Bacterial expression of GST-tagged p37 lacking the UBX domain

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    delta 251-331 (stop codon behind AA250)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. hKLF6 P148L (1009)

    Plasmid

    #49497

    Purpose

    expresses human KLF6 with P148L mutation

    Depositor

    Scott Friedman

    Article

    Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

    Insert

    KFL6-P148L (KLF6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P148L

    Promoter

    CMV

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pPHR-EYFP-NLS-VP16

    Plasmid

    #103822

    Purpose

    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-EYFP-VP16 (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VP16: G126T (silent, G42G)

    Promoter

    CMV

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLIK 3XFLAG-MKK7-EE-NLS hygro

    Plasmid

    #87780

    Purpose

    Inducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK7 (Map2k7 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

19. Gal4-VP16-HSF1Δ∼PRD

    Plasmid

    #71727

    Purpose

    plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RD

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    Gal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Budding Yeast, Herpes Simplex Virus protein VP16, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac1-ScVip1-536-1107-delta848-918-R547A-H548A-R551A

    Plasmid

    #238314

    Purpose

    ScVip1-536-1107-delta848-918-R547A-H548A-R551A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-delta848-918-R547A-H548A-R551A

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBB3-ScVip1-536-1107-E576A-K623A-Q625S-K627A

    Plasmid

    #238327

    Purpose

    ScVip1-536-1107-E576A-K623A-Q625S-K627A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-E576A-K623A-Q625S-K627A

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBB3-ScVip1-536-1107-K558A-K605A-K732A-K817A

    Plasmid

    #238325

    Purpose

    ScVip1-536-1107-K558A-K605A-K732A-K817A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-K558A-K605A-K732A-K817A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYO379

    Plasmid

    #235733

    Purpose

    Protein expression of VPS34 + VPS15-ZZ + ZZ-VPS30 (all yeast) in yeast

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Inserts

    VPS34 (VPS34 Budding Yeast)
    VPS15 (VPS15 Budding Yeast)
    VPS30 (VPS30 Budding Yeast)
    

    Use

    Tags

    3xTEV-ZZ and ZZ-3xTEV

    Expression

    Yeast

    Mutation

    S2A on ScVPS34

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOP795

    Plasmid

    #235700

    Purpose

    Protein expression His-taged HsNRBF2_FL in bacteria

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    NRBF2 (NRBF2 Human)
    

    Use

    Tags

    His6(TEV)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOP796

    Plasmid

    #235701

    Purpose

    Protein expression of His-tagged HsNRBF2 MIT domain in bacteria

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    NRBF2 MIT domain (NRBF2 Human)
    

    Use

    Tags

    His6(TEV)

    Expression

    Bacterial

    Mutation

    aa 1-89

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p413 TEF NAPstar1

    Plasmid

    #232738

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar1 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p413 TEF NAPstar2

    Plasmid

    #232739

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar2 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p413 TEF NAPstar4

    Plasmid

    #232741

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar4 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p413 TEF NAPstar6

    Plasmid

    #232742

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar6 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p413 TEF NAPstar7

    Plasmid

    #232744

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar7 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar7
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge