Addgene

We narrowed to 4,281 results for: ARA-2

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Nsp13 deltaN2-EGFP

Plasmid

#165128

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-2_M13ps

Plasmid

#231412

Purpose

M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2-mt2*

Plasmid

#220246

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2* (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

PRKKKR494-499NAAIRS

Promoter

EF-1α

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBS L30 Ruby3-Rab7A

Plasmid

#135651

Purpose

Rab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab7A (RAB7A Human)

Use

Tags

Ruby3

Expression

Mammalian

Mutation

Promoter

L30

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EZH2-mt2

Plasmid

#220240

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Use

Tags

6x His, MBP

Expression

Insect

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

Polyhedrin

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2-mt2

Plasmid

#220245

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

EF-1α

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFl Strep-sumo-TEV-hAgo2 D597N

Plasmid

#136236

Purpose

Expressing catalytically inactive human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

Insert

human Argonaute-2 (AGO2 Human)

Use

Tags

Sterp Sumo

Expression

Insect

Mutation

D597N

Promoter

polyhedrin

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBS L30 Flag-Apex-Rab7A

Plasmid

#135652

Purpose

Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab7A (RAB7A Human)

Use

Tags

Apex2 and FLag

Expression

Mammalian

Mutation

Promoter

L30

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pKMyc

Plasmid

#19400

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Type

Empty backbone

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2009

Availability

Academic Institutions and Nonprofits only

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pHM103

Plasmid

#225527

Purpose

Encodes constitutive GFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Insert

GFP

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSBK.010

Plasmid

#187210

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 2 [GCT-AGC]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 2 [GCT-AGC])
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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915-Plakophilin 2a-Flag

Plasmid

#32230

Depositor

Kathleen Green

Article

Chen et al J Biol Chem. 2002 Mar 22;277(12):10512-2

Insert

Plakophilin 2a (PKP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3 S6K2 WT

Plasmid

#17729

Depositor

John Blenis

Article

Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

Insert

S6K2 (RPS6KB2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pFlag-CMV2-WBP2

Plasmid

#27478

Depositor

Marius Sudol

Article

Chen et al J Biol Chem. 1997 Jul 4. 272(27):17070-7

Insert

WW domain binding protein-2 (WBP2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
CIPK2-upGFP

Plasmid

#117863

Purpose

protein expression

Depositor

Geoffrey Chang

Insert

CIPK2-upGFP (CIPK2 Mustard Weed)

Use

Tags

eGFP

Expression

Yeast

Mutation

Promoter

AOX

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry PGK-1 in pcDNA3.1

Plasmid

#64214

Purpose

mCherry fused between Cry2 and PGK-1 to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
PGK1 (PGK1 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

Plasmid

#64207

Purpose

mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 (CRY2 Mustard Weed)
RGS4(del 1-33) (RGS4 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pAc5.1B-lambdaN-DmPABPC1-siRNAres-V5His6_I

Plasmid

#146586

Purpose

Insect Expression of DmPABPC1-siRNAres

Depositor

Elisa Izaurralde

Article

Nishihara et al Nucleic Acids Res. 2013 Oct;41(18):8692-

Insert

DmPABPC1-siRNAres (pAbp Fly)

Use

Tags

Expression

Insect

Mutation

seven silent mutations compared to the sequence g…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 S6K2 KR

Plasmid

#17730

Depositor

John Blenis

Article

Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

Insert

S6K2 (RPS6KB2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K99R: kinase dead mutant

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmNot2

Plasmid

#79255

Purpose

Expresses EGFP-tagged DmNot2 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmNot2 (Rga Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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