We narrowed to 2,376 results for: LARGE;

1. attB-puro donor.dna

   Plasmid

   #181923

   Purpose

   attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attB-EGFP-EF1α-PuroR-T2A-TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. attP-puro donor

   Plasmid

   #182139

   Purpose

   attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attP-EGFP-EF1α-PuroR-T2A-TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE2-TetOminiCMV-SV40LT

   Plasmid

   #136616

   Purpose

   Dox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cells

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   SV40 large T antigen
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEG BacMam

   Plasmid

   #160451

   Purpose

   vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA5/FRT-ChemoG-NAD

   Plasmid

   #193821

   Purpose

   CMV overexpression of ChemoG-NAD in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG-NAD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K, T225R; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA5/FRT-ChemoG-CaM

   Plasmid

   #193816

   Purpose

   CMV overexpression of ChemoG-CaM in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG-CaM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:E116D, A206K; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA5/FRT-ChemoG-ATP

   Plasmid

   #193818

   Purpose

   CMV overexpression of ChemoG-ATP in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG-ATP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA5/FRT-ChemoB-ATP

   Plasmid

   #193819

   Purpose

   CMV overexpression of ChemoB-ATP in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoB-ATP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EBFP2:N39Y, A206K; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA5/FRT/TO-[4xCox8]-ChemoG-NAD

   Plasmid

   #193823

   Purpose

   CMV overexpression of ChemoG-NAD in the mitochondria of mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   [4xCox8]-ChemoG-NAD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K, T225R; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA5/FRT-ChemoL-CaM

    Plasmid

    #193830

    Purpose

    CMV overexpression of ChemoL-CaM in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoL-CaM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP:A206K, E299D; HT7: L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSc-humanADAR2

    Plasmid

    #171106

    Purpose

    Expresses human ADAR2 in yeast.

    Depositor

    Brenda Bass

    Article

    Macbeth et al Methods Enzymol. 2007;424:319-31. doi: 1

    Insert

    human ADAR2 (ADARB1 Human)
    

    Use

    Tags

    His12_TEV

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA6 MCV LTco

    Plasmid

    #40200

    Depositor

    Patrick Moore

    Article

    Shuda et al J Clin Invest. 2011 Sep;121(9):3623-34.

    Insert

    MCPyV LT codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA6 MCV LTco S220A

    Plasmid

    #112193

    Purpose

    Expression of MCPyV LT mutant (Ser 220 to Ala)

    Depositor

    Patrick Moore

    Article

    Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

    Insert

    MCPyV LT codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA5/FRT-ChemoL-NAD

    Plasmid

    #193827

    Purpose

    CMV overexpression of ChemoL-NAD in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoL-NAD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AY27_pU6.gRNA.CLYBL

    Plasmid

    #199238

    Purpose

    Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    S. pyogenes gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-2(KW)2-mCherry

    Plasmid

    #112738

    Purpose

    Express 2(KW)2-mCherry in E. coli

    Depositor

    Kyubong Jo

    Article

    Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

    Insert

    2(KW)2-mCherry
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. AD59_pEP.DonorCLYBL.TS

    Plasmid

    #199227

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    

    Use

    Gene targeting donor plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1 gene promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA5/FRT-ChemoC

    Plasmid

    #193809

    Purpose

    CMV overexpression of ChemoC in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoC
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    mCerulean3:T225R; HT7: E143R, E147R, L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQAT03

    Plasmid

    #223375

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge