We narrowed to 2,098 results for: Pam

1. xCas9 3.7

   Plasmid

   #108379

   Purpose

   Mammalian expression vector for xCas9 3.7

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9 3.7
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

   Plasmid

   #194063

   Purpose

   Mammalian expression of evoCjCas9 PEmax_deltaRnH

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-PEmax_dRnH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Nme2Cas9_pMCSG7

   Plasmid

   #119925

   Purpose

   Nme2Cas9 bacterial expression plasmid

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   Tags

   NLS, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCnCas12f1HS_sgRNA_MS13_empty

   Plasmid

   #204999

   Purpose

   CnCas12f1 with MS13 sgRNA site for Human cell genome editing

   Depositor

   Quanjiang Ji

   Article

   Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PE2-SpRY-P2A-GFP

   Plasmid

   #159981

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpRY-P2A-GFP
   

   Use

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2T-CMV-evoCjCas9-ABE8e-BlastR

   Plasmid

   #194060

   Purpose

   Mammalian expression of evoCjCas9 ABE8e

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-ABE8e
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-P3-evoCjCas9-ABE8e_gRNA

   Plasmid

   #202559

   Purpose

   P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-ABE8e
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-eNme2-C.NR

   Plasmid

   #185672

   Purpose

   Expresses nuclease active eNme2-C.NR in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   eNme2-C.NR nuclease
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

   Promoter

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTW211b5

   Plasmid

   #136927

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9(N)-NpuN
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   see manuscript

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-PE2-SpG-P2A-GFP

    Plasmid

    #159980

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpG-P2A-GFP
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-Cas9-NRCH

    Plasmid

    #136926

    Purpose

    Expresses Cas9-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a_CnCas12f1

    Plasmid

    #204996

    Purpose

    CnCas12f1 heterologous expression plasmid

    Depositor

    Quanjiang Ji

    Article

    Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

    Insert

    CnCas12f1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEND-418_Cacnes_LysA

    Plasmid

    #225607

    Purpose

    Suicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nme2sgRNA_pLKO

    Plasmid

    #119926

    Purpose

    Nme2Cas9 U6-driven sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    NmeCas9 sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. xCas9 3.6

    Plasmid

    #108384

    Purpose

    Mammalian expression vector for xCas9 3.6

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9 3.6
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-SlugABE-NNG

    Plasmid

    #214356

    Purpose

    Expresses SlugABE-NNG

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2T-CMV-evoCjCas9-BE4max-BlastR

    Plasmid

    #194061

    Purpose

    Mammalian expression of evoCjCas9 CBE4max

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-hSyn-evoCjCas9-ABE8e_gRNA

    Plasmid

    #202561

    Purpose

    hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-ABE8e
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Cas9-NRRH

    Plasmid

    #136924

    Purpose

    Expresses Cas9-NRRH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRRH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge