We narrowed to 59,251 results for: cloning

1. pD(Ct)_3C-TS

   Plasmid

   #223892

   Purpose

   4G-cloning donor for C-terminal 3C-TwinStrep tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3C-TwinStrep
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD(Gib)_5'alpha

   Plasmid

   #223863

   Purpose

   4G-cloning donor for 5'-Gibson overhang, alpha-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   5'alpha sequence and spacer
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD(Gib)_5'gamma

   Plasmid

   #223865

   Purpose

   4G-cloning donor for 5'-Gibson overhang, gamma-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   5'gamma sequence and spacer
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD(Gib)_5'beta

   Plasmid

   #223864

   Purpose

   4G-cloning donor for 5'-Gibson overhang, beta-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   5'beta sequence and spacer
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD(Gib)_5'delta

   Plasmid

   #223866

   Purpose

   4G-cloning donor for 5'-Gibson overhang, delta-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   5'delta sequence and spacer
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD(Gib)_3'beta

   Plasmid

   #223867

   Purpose

   4G-cloning donor for 3'-Gibson overhang, beta-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3'beta sequence and spacer
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pD(Gib)_3'gamma

   Plasmid

   #223868

   Purpose

   4G-cloning donor for 3'-Gibson overhang, gamma-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3'gamma sequence and spacer
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD(Gib)_3'delta

   Plasmid

   #223869

   Purpose

   4G-cloning donor for 3'-Gibson overhang, delta-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3'delta sequence and spacer
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p663-UBC-MTS-miniTurbo-V5_IDG-K

   Plasmid

   #214456

   Purpose

   Gateway destination clone of MTS-miniTurbo (mitochondrial control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   MTS-miniTurbo-V5
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p663-UBC-ER-miniTurbo-V5_IDG-K

    Plasmid

    #214452

    Purpose

    Gateway destination clone of ER-miniTurbo (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ER-miniTurbo-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p663-UBC-MTS-TurboID-V5_IDG-K

    Plasmid

    #214457

    Purpose

    Gateway destination clone of MTS-TurboID (mitochondrial control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    MTS-TurboID-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGGW_SM_BglII

    Plasmid

    #49314

    Purpose

    Subcloning vector for Golden Gate-based site-directed mutagenesis; BglII and KpnI to release the insert

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-mRFP

    Plasmid

    #13032

    Purpose

    Mammalian expression vector for cloning your gene fused to mRFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Monomer Red Fluorescent Protein
    

    Expression

    Mammalian

    Available Since

    Nov. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-YFP

    Plasmid

    #13033

    Purpose

    Mammalian expression vector for cloning your gene fused to YFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Yellow fluorescent protein
    

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a- gfp-2a-puro

    Plasmid

    #129443

    Purpose

    expression of GFP and Puro R

    Depositor

    Oskar Laur

    Insert

    gfp-puro
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-CMV-mCherry-EF1 Hygro

    Plasmid

    #129440

    Purpose

    express mCherry

    Depositor

    Oskar Laur

    Insert

    mcherry
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28GSTlinker-LIC

    Plasmid

    #119970

    Purpose

    Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. 60-p3E_INTRON-EmptyRNAi

    Plasmid

    #163380

    Purpose

    p3E-Vector (Gateway R2-L3) for Tol2-kit cloning. Vector for performing RNAi (gene silencing - knockdown) in zebrafish via cloning and expression of synthetic miR(s).

    Depositor

    Jean Giacomotto

    Type

    Empty backbone

    Use

    RNAi

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-UBC-LUC-EF1 Hygro

    Plasmid

    #129436

    Purpose

    express LUC

    Depositor

    Oskar Laur

    Insert

    LUC
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. R777-E211 Hs.RAC3

    Plasmid

    #70495

    Purpose

    Gateway ORF clone of human RAC3 [NM_005052.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RAC3 (RAC3 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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