We narrowed to 18,335 results for: met

1. peT3a-AqAdk_MVGDH

   Plasmid

   #18092

   Depositor

   Dorothee Kern

   Article

   Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

   Insert

   adenylate kinase (kad A. aeolicus)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Tyr 52 changed to Cys, Val 145 changed to Cys, …

   Promoter

   Available Since

   May 29, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-T7-null (BPK1077)

   Plasmid

   #171128

   Purpose

   Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

   Depositor

   Benjamin Kleinstiver

   Article

   Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

   Insert

   pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   pCMV and T7

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3XFlagNICD3

   Plasmid

   #20185

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 3 Intracellular Domain (Notch3 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Contains murine Notch 3 Intracellular Domain (Met…

   Promoter

   CMV

   Available Since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-RO1.7-RPE65

   Plasmid

   #206346

   Purpose

   AAV plasmid expressing RPE65 in photoreceptors

   Depositor

   Connie Cepko

   Article

   Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

   Insert

   Rpe65 (Rpe65 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   450-Met (C57BL6 natural variation)

   Promoter

   1.7kb human red opsin

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3XFlagNICD2

   Plasmid

   #20184

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 2 intracellular domain (Notch2 Mouse)
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Contains murine Notch 2 Intracellular Domain (Met…

   Promoter

   CMV

   Available Since

   Jan. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET23-PBD(65-150)-N-Cys

   Plasmid

   #13722

   Depositor

   Klaus Hahn

   Article

   Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

   Insert

   PBD(65-150)-N-Cys (PAK1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Met-Cys-Gly-PBD(65-150)-

   Promoter

   Available Since

   March 28, 2007

   Availability

   Academic Institutions and Nonprofits only

7. Erk2

   Plasmid

   #40777

   Purpose

   Expression of rat Erk2 in mammalian cells

   Depositor

   Dustin Maly

   Article

   Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

   Insert

   Erk2 (Mapk1 Rattus norvegicus)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Starting Met removed

   Promoter

   CMV

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

8. YFP(1-158) in pcDNAI/Amp

   Plasmid

   #60888

   Purpose

   This vector attaches YFP(1-158) to the N-terminus of a protein.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(1-158)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met was substituted for Gln-69.

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBW105

   Plasmid

   #111137

   Purpose

   For the expression of rat AANAT with Met-Leu-Ser N-terminus in S. cerevisiae

   Depositor

   Alexander Varshavsky

   Article

   Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

   Insert

   MLS-ratAANAT-3FLAG (Aanat Rat)
   

   Use

   Tags

   3x Flag

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Rtn2b-RHD272-472-GFP

    Plasmid

    #186606

    Purpose

    Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFP

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Met-Gly-Ser-Lys peptide added at N-terminus for c…

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Rtn2b-RHD272-472-mNeon

    Plasmid

    #186605

    Purpose

    Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Met-Gly-Ser-Lys peptide added at N-terminus for c…

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET51b(+)_EGFP-LAMA-S98 (for p24)

    Plasmid

    #130717

    Purpose

    Plasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid protein

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-LAMA-S98
    

    Use

    Tags

    Hisx10 and StrepTag

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW106

    Plasmid

    #111138

    Purpose

    For the expression of rat AANAT with Met-Ser-Ile N-terminus in S. cerevisiae

    Depositor

    Alexander Varshavsky

    Article

    Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

    Insert

    MSI-ratAANAT-3FLAG (Leu2 deletion) (Aanat Rat)
    

    Use

    Tags

    3x Flag

    Expression

    Yeast

    Mutation

    Leu2 deletion

    Promoter

    Gal1

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM6_Ptrc_BsMdh_BmHps_BmPhi

    Plasmid

    #129104

    Purpose

    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

    Insert

    BsMDH BmHPS BmPHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pDCAF3-IS5

    Plasmid

    #65220

    Purpose

    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

    Depositor

    Jeffrey Barrick

    Article

    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

    Inserts

    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed start codon from gtg to atg and Changed s…

    Promoter

    BBa_J23100

    Available Since

    Jan. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

    Plasmid

    #129105

    Purpose

    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

    Insert

    BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pCMV-dCas13-inactive M3M14nes

    Plasmid

    #157855

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Promoter

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBK- Ma PylRS WT

    Plasmid

    #197573

    Purpose

    Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Insert

    M. alvus PylRS WT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    GlnS (constitutive)

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pALS2-sfGFP WT

    Plasmid

    #197575

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    M. alvus Pyl-tRNA (6)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    araC and lpp

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAcBac1-Ma-sfGFP WT

    Plasmid

    #197567

    Purpose

    Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    M. alvus Pyl-tRNA (6) (4x copies)
    

    Use

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6/H1

    Available Since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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