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pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

Plasmid

#140201

Purpose

Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.

Depositor

Yit Heng Chooi

Publication

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Cloning of crrna an…

Tags

dLbCas12a crRNA scaffold

Promoter

PgpdA

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12R

Plasmid

#82804

Purpose

Gateway Donor vector containing KRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G12R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5L18-CtermQ7

Plasmid

#51442

Purpose

Expresses TALEN targeting left CCR5A site (L18) with Q7 C-terminal domain

Depositor

David Liu

Publication

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) Q7 C-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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BPK1520_blastR

Plasmid

#175289

Purpose

Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection

Depositor

Ronald Cohn

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV/U6

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285

Plasmid

#31060

Depositor

Gerhart Ryffel

Publication

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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57F03_LexA-GAD

Plasmid

#83256

Purpose

Expresses LexA-GAD under the control of 57F03 enhancer

Depositor

Leslie Vosshall

Publication

Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

Insert

57F03 enhancer

Expression

Insect

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-AviTag

Plasmid

#67175

Purpose

CRISPaint universal donor for AviTag fusion tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

AviTag

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pRVdG-4tdTomato

Plasmid

#52500

Purpose

Expresses tdTomato

Depositor

Sebastian Seung

Insert

tdTomato

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLenti-puro/RAF1-S259A

Plasmid

#131727

Purpose

Mammalian Expression of RAF1

Depositor

Jacques De Grève

Publication

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S259A

Promoter

CMV

Available since

May 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_RALA_WT_V5

Plasmid

#82988

Purpose

Gateway Donor vector containing RALA, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RALA (RALA Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pYFAC-pyroA

Plasmid

#168976

Purpose

Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; pyroA

Depositor

Yit Heng Chooi

Publication

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus nidu…

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_RARA_WT_V5

Plasmid

#83018

Purpose

Gateway Donor vector containing RARA , used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RARA (RARA Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_ID2_WT_V5

Plasmid

#82960

Purpose

Gateway Donor vector containing ID2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ID2 (ID2 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RARA_WT

Plasmid

#82886

Purpose

Gateway Donor vector containing RARA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_001145301 (RARA Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZBTB24_WT

Plasmid

#82887

Purpose

Gateway Donor vector containing ZBTB24, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZBTB24 (ZBTB24 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFGL822

Plasmid

#58225

Purpose

For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.

Depositor

Naweed Naqvi

Publication

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

phosphinothricin acetyl transferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223_KEAP1_p.R470S

Plasmid

#82781

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway Cloning

Mutation

R470S; C624S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAG426Gal-GFP

Plasmid

#38310

Depositor

Susan Lindquist

Publication

McLellan et al ACS Chem Biol. 2012 Jun 22.

Insert

EGFP (eGFP )

Expression

Yeast

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-PM mir shLEF1 a

Plasmid

#27019

Depositor

Joan Massague

Publication

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir LEF1 3a

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pV1435

Plasmid

#111439

Purpose

Solo vector pV1382 + sgCgADE2

Depositor

Gerald Fink

Publication

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgCgADE2

Use

CRISPR

Expression

Yeast

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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