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Addgene

We narrowed to 9,203 results for: sacs

Showing: 7771 - 7800 of 9203 results
  1. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3

    Plasmid
    #221112
    Purpose
    FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Insert
    MFA-IgKappa-FAPoptim-STE3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    STE3
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. PDG458 eSp(1.1) V3

    Plasmid
    #226964
    Purpose
    CBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pET28_6xHis-GAL4-PARP1-CAT-L713F

    Plasmid
    #175949
    Purpose
    Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation
    Depositor
    Insert
    GAL4 DNA binding domain (GAL4 Budding Yeast)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    L713F, V762A for the 'PARP1-CAT-L713F' …
    Promoter
    T7
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pIRHDGAL43C

    Plasmid
    #218279
    Purpose
    Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction
    Depositor
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
    Use
    Tags
    Expression
    Yeast
    Mutation
    GAL3 (S509P), DHFR (P66L)
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pIRHDGAL3C

    Plasmid
    #218276
    Purpose
    Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction
    Depositor
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
    Use
    Tags
    Expression
    Yeast
    Mutation
    GAL3 (S509P), DHFR (P66L)
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. Coexpressed VLP NES

    Plasmid
    #182433
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Inserts
    VP2C-FPPS
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, GAL10, and GAL7
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. Coexpressed VLP NES (M)

    Plasmid
    #182435
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Inserts
    VP2C-FPPS(M)
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, GAL10, and GAL7
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. Coexpressed VLP NES (G)

    Plasmid
    #182477
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Inserts
    VP2C-FPPS(G)
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, GAL10, and GAL7
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 7771 - 7800 of 9203 results