We narrowed to 9,203 results for: sacs
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Plasmid#221112PurposeFAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ERDepositorInsertMFA-IgKappa-FAPoptim-STE3
UseTagsExpressionYeastMutationPromoterSTE3Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETcon-pGAL1-Aga2-α-Synuclein-c_myc
Plasmid#225222PurposeYeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.DepositorInsertsα-Synuclein
Aga2
UseTagsGS linker, HA Tag, and c-myc TagExpressionYeastMutationPromoterGAL1Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTT021
Plasmid#226782PurposeExpression of wzxE and other genes from the E. coli enterobacterial common antigen biosynthesis gene clusterDepositorInsertwzxE, wecF, wzyE, wecG
UseTagsExpressionBacterialMutationPromoterBADAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
PDG458 eSp(1.1) V3
Plasmid#226964PurposeCBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1(tandem S131C-wt)
Plasmid#224777PurposeMammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linkerDepositorInsertTandem TREK-1 (S131C-wt) (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationS131CPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1(S131C/A286F)
Plasmid#224775PurposeMammalian expression vector for generating mouse TREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationS131C / A286FPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1(tandem wt-S131C)
Plasmid#224778PurposeMammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linkerDepositorInsertTandem TREK-1 wt-S131C) (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationS562CPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1(tandem wt-wt)
Plasmid#224776PurposeMammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linkerDepositorInsertTandem TREK-1 (wt-wt) (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(tandem S131C-S131C)
Plasmid#224760PurposeIn vitro transcription for Oocyte expression of mTREK-1(tandem S131C-S131C)DepositorInsertTandem TREK-1 (S131C-S131C) (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131C / S562CPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (S131A/C159A/C219A)
Plasmid#224753PurposeIn vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131A/C159A/C219APromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (S131C/C159A/C219A)
Plasmid#224752PurposeIn vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131C/C159A/C219APromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A)
Plasmid#224754PurposeIn vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationC159A/C219A/C365A/C399APromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) C90A, T172C
Plasmid#217983Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationC90A, T172CPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-GAL4-PARP1-CAT-L713F
Plasmid#175949PurposeBacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutationDepositorInsertGAL4 DNA binding domain (GAL4 Budding Yeast)
UseTags6xHis tagExpressionBacterialMutationL713F, V762A for the 'PARP1-CAT-L713F' …PromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) T160C
Plasmid#220233Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationT160CPromoterT7Available sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(85-160)
Plasmid#217984Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-100)
Plasmid#217978Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6GB1-CREB(1-280) C90A
Plasmid#217982Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationC90APromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) A50C,C90A
Plasmid#217985Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationA50C, C90APromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280)
Plasmid#217981Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(160-280)
Plasmid#217980Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRHDGAL43C
Plasmid#218279PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
UseTagsExpressionYeastMutationGAL3 (S509P), DHFR (P66L)PromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRHDGAL3C
Plasmid#218276PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
UseTagsExpressionYeastMutationGAL3 (S509P), DHFR (P66L)PromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP
Plasmid#212795PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeastDepositorInsertHis6-CUbo(K48R/K63R)-GFP
UseIntegrative vectorTagsExpressionYeastMutationCUb(K48R/K63R)PromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pROS_phleo-Tps1/Gsy2
Plasmid#196612PurposeEncoding guide RNAs for the knock out of TPS1 and GSY2 genesDepositorUseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROS13-Tps2/Gsy1
Plasmid#196613PurposeEncoding guide RNAs for the knock out of TPS2 and GSY1 genesDepositorUseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUGP1.1
Plasmid#196615PurposePlasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeastDepositorInsertUGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES
Plasmid#182433PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertsVP2C-FPPS
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES (M)
Plasmid#182435PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES (G)
Plasmid#182477PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only