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pAAV-FLEXfrt-SaCas9-U6-sgGabrg3

Plasmid

#124872

Purpose

Mutagenesis of Gabrg3

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg3 (Gabrg3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg2

Plasmid

#124871

Purpose

Mutagenesis of Gabrg2

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg2 (Gabrg2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJB040 pAAV EF1a Nuc-flox(mCherry)-mNeonGreen

Plasmid

#252772

Purpose

An AAV vector that expresses a Cre-dependent nuclear-localized mCherry to mNeonGreen

Depositor

Joshua Leonard

Publication

Boucher et al J Extracell Vesicles. 2026 Apr;15(4):e70

Insert

Nuc-flox(mCherry)-mNeonGreen

Use

AAV

Promoter

hEF1a

Available since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA

Plasmid

#191148

Purpose

AAV expression of a Cre reporter with EYFP output

Depositor

Xiaojing Gao

Publication

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

DIO-EYFP

Use

AAV

Expression

Mammalian

Mutation

WT

Promoter

hEF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

Plasmid

#185717

Purpose

AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

Depositor

Jun Ding

Publication

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Mutation

Changed Ala 11 to Pro, Val 70 to Pro

Promoter

hSyn

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-HBA-RIBEYE-eGFP

Plasmid

#241988

Purpose

Human RIBEYE cDNA cloned inframe into a pAAV-GFP vector driven by hybrid CMV-enhancer-human-beta-actin promoter (CMV-HBA) and containing downstream WPRE, Ampicillin Resistance

Depositor

Tobias Moser

Publication

Kapoor et al Elife. 2026 Jan 13;13:RP98254. doi: 10.7

Insert

RIBEYE-GFP (CTBP2 Human)

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

hybrid CMV-enhancer-human-beta-actin promoter (CM…

Available since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ef1a-fDIO-mRuby3-2A-dCre

Plasmid

#203845

Purpose

Flp-dependent enzymatically inactive Cre and mRuby3 expression

Depositor

Ivo Spiegel

Publication

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dCre

Use

AAV

Tags

mRuby3-2A

Mutation

Enzymatically inactive Cre

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SuperNovaGreen-T2A-dTomato

Plasmid

#193798

Purpose

Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

Depositor

Xiao Wang

Publication

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

SuperNova Green

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FRT-EBFP-tTAn

Plasmid

#172127

Purpose

Expression of EBFP and tTA-InteinN in Flp recombinase positive cells

Depositor

Chun Xu

Publication

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EBFP, tTAn-InteinN

Use

AAV

Promoter

EF1a

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

Plasmid

#127865

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Publication

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

deleted the c-terminal 20 amino acids

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE

Plasmid

#127868

Purpose

pAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Publication

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1 (Nos1 Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Amino acids 1-133 only

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pX459-sgAAVS1

Plasmid

#184403

Purpose

pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

Depositor

Robert Bradley

Publication

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AAVS1-specific sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAGGS-Flex/3'USS(103)-GFP

Plasmid

#197890

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Publication

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(285)-GFP

Plasmid

#197889

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Publication

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244071

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244072

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-mGreenLantern

Plasmid

#220596

Purpose

Constitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.

Depositor

Sam Golden

Publication

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mGreenLantern

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-SpdCas9-W3SL

Plasmid

#210708

Purpose

This Plasmid express hSyn promoter driven SpdCas9

Depositor

Isaac Hilton

Publication

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

S. Pyogenes dCas9

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

D10A/H840A

Promoter

hSyn

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)

Plasmid

#156164

Purpose

Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequence

Depositor

Kevin Park

Publication

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with addition of ER-retention sequence KDEL

Promoter

CMV

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hSncg-EGFP

Plasmid

#153198

Purpose

Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Publication

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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