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pME Actin Vhh Halo Tag (JDW 1223)

Plasmid

#224498

Purpose

Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-Halo-Tag

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-SmBiT

Plasmid

#223618

Purpose

Lentiviral expression of human CD86 with SmBiT tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SmBiT

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-mTFP1

Plasmid

#223606

Purpose

Lentiviral expression of human CD86 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyB-GFP

Plasmid

#220190

Purpose

sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG35

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyC-GFP

Plasmid

#220191

Purpose

sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-PLIN3

Plasmid

#222420

Purpose

To express a lipid droplet "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Publication

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PLIN3 (PLIN3 Human)

Tags

FRB-mCherry

Expression

Mammalian

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(FL)

Plasmid

#221243

Purpose

Expression of STING without transmembrane domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING without transmembrane domain (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGM8

Plasmid

#220166

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Publication

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TSPO (TSPO Human)

Use

Lentiviral

Tags

GFP11

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA5-PSMA6-PSMA7

Plasmid

#217894

Purpose

Insect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)

Depositor

Brenda Schulman

Publication

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA5 (PSMA5 Human)
PSMA6 (PSMA6 Human)
PSMA7 (PSMA7 Human)

Expression

Insect

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-SARS-CoV-2-M

Plasmid

#215813

Purpose

Expresses SARS-CoV-2 Membrane protein in mammalian cells

Depositor

Thomas Gallagher

Publication

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 Membrane (M )

Expression

Mammalian

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

Plasmid

#195049

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pPGI

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 HA pCAGGS

Plasmid

#206107

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549

Depositor

Annette Dolphin

Publication

Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Promoter

CMV/B-actin

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-PIP4K2AD273K

Plasmid

#202727

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mTagBFP2:PIP4K2A(D266K) (PIP4K2A Human)

Expression

Mammalian

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2- PIP4K2BD278K

Plasmid

#202729

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mTagBFP2:PIP4K2B (D278K) (PIP4K2B Human)

Expression

Mammalian

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyG1_zeo

Plasmid

#184910

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Dcst2-3xHA

Plasmid

#183543

Purpose

Expression vector of mouse DC-STAMP domain containing 2 (Dcst2) tagged with 3xHA at C-terminus.

Depositor

Masahito Ikawa

Publication

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DC-STAMP domain containing 2 (Dcst2 Mouse)

Tags

HA

Expression

Mammalian

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmbim6-V5

Plasmid

#175150

Purpose

Lentiviral expression of mouse Tmbim6-V5

Depositor

Larry Gerace

Publication

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmbim6 (Tmbim6 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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mRuby2-AKTIP

Plasmid

#171942

Purpose

Expresses AKTIP in mammalian cells

Depositor

Cheng-han Yu

Publication

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AKTIP (AKTIP Human)

Tags

mRuby2

Expression

Mammalian

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E45: attB4-hr5-ie1p>-attB1r

Plasmid

#162930

Purpose

Gateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPV

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV ie1 promoter with hr5 enhancer

Use

Synthetic Biology

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pKI-cjVASA-tdTomato

Plasmid

#160872

Purpose

Marmoset Targeting

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tdTomato

Use

Marmoset targeting

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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