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MSCV-Neo-GFP/Flag-Gata5

Plasmid

#105508

Purpose

MSCV-driven retroviral Gata5 expression (Flag-tagged)

Depositor

Christopher Vakoc

Publication

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Gata5 (Gata5 Mouse)

Use

Retroviral

Tags

Flag

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST mito roGFP2-Orp1

Plasmid

#64997

Purpose

for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Publication

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MXS_PGK::PuroR-bGHpA

Plasmid

#62440

Purpose

MXS_chaining vector with PGK::PuroR-bGHpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with PGK Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::ZeoR-bGHpA

Plasmid

#62442

Purpose

MXS_chaining vector with PGK::ZeoR-bGHpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with PGK Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA4_MYOD1

Plasmid

#64139

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA1_MYOD1

Plasmid

#64136

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA2_MYOD1

Plasmid

#64137

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pPL5628_TEF1*-Cre_MET15

Plasmid

#60933

Purpose

Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

Depositor

Robert Piper

Publication

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Promoter

mutant TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLJM60 mUlk1 (wt) barcoded

Plasmid

#48799

Purpose

expresses wt Ulk1

Depositor

David Sabatini

Publication

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

ULK1 (Ulk1 Mouse)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Promoter

CMV

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-GluCl_cryst

Plasmid

#31487

Depositor

Eric Gouaux

Publication

Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.

Insert

C. elegans glutamate-gated chloride channel alpha, crystallized construct

Use

For rna synthesis for expression in x. laevis ooc…

Tags

8x-His

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-RPS6KA6

Plasmid

#23540

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KA6 (RPS6KA6 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PANK2

Plasmid

#23583

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PANK2 (PANK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MYLK2

Plasmid

#23656

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MYLK2 (MYLK2 Human)

Use

Gateway Cloning

Mutation

P86A

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP2K6

Plasmid

#23783

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP2K6 (MAP2K6 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKACG

Plasmid

#23788

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKACG (PRKACG Human)

Use

Gateway Cloning

Mutation

1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
OPEN HLA-A*02:01-BirA

Plasmid

#215569

Purpose

E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Publication

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN HLA-A*02:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

Glycine 120 to Cysteine

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pTET2-RlucCP

Plasmid

#198352

Purpose

Part of Firefly reporter system, encodes Renilla luciferase

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

pTET2

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-trAtCCD7-OsD27(pYL726 )

Plasmid

#178285

Purpose

Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

OsD27
trAtCCD7

Expression

Bacterial

Promoter

T7

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-gp130dimer

Plasmid

#174394

Purpose

Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

Depositor

Dennis Ko

Publication

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IL6ST cytosolic domain with leucine zipper & C-C linker

Tags

Flag

Expression

Mammalian

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-ECFP-Actb

Plasmid

#169787

Purpose

An actin FRET sensor. Must be expressed with Venus-actin for FRET.

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Actb (Actb Mouse)

Tags

ECFP

Expression

Mammalian

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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