We narrowed to 59,446 results for: cloning

1. pcDNA3.1 Flag YFP hNKCC1 L671C (NT440)

   Plasmid

   #50872

   Purpose

   Expresses human NKCC1 L671C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   L671C in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Flag YFP hNKCC1 E670C (NT473)

   Plasmid

   #50871

   Purpose

   Expresses human NKCC1 E670C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   E670C in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUSC8

   Plasmid

   #50428

   Purpose

   Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 8.5 RVDs long.

   Depositor

   Andrew Scharenberg

   Article

   Boissel et al Nucleic Acids Res. 2013 Nov 26.

   Type

   Empty backbone

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUSC7

   Plasmid

   #50427

   Purpose

   Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 7.5 RVDs long.

   Depositor

   Andrew Scharenberg

   Article

   Boissel et al Nucleic Acids Res. 2013 Nov 26.

   Type

   Empty backbone

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUSC6

   Plasmid

   #50426

   Purpose

   Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 6.5 RVDs long.

   Depositor

   Andrew Scharenberg

   Article

   Boissel et al Nucleic Acids Res. 2013 Nov 26.

   Type

   Empty backbone

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUSC5

   Plasmid

   #50425

   Purpose

   Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 5.5 RVDs long.

   Depositor

   Andrew Scharenberg

   Article

   Boissel et al Nucleic Acids Res. 2013 Nov 26.

   Type

   Empty backbone

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUSC4

   Plasmid

   #50424

   Purpose

   Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 4.5 RVDs long.

   Depositor

   Andrew Scharenberg

   Article

   Boissel et al Nucleic Acids Res. 2013 Nov 26.

   Type

   Empty backbone

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUSC3

   Plasmid

   #50423

   Purpose

   Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 3.5 RVDs long.

   Depositor

   Andrew Scharenberg

   Article

   Boissel et al Nucleic Acids Res. 2013 Nov 26.

   Type

   Empty backbone

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUSC2

   Plasmid

   #50422

   Purpose

   Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 2.5 RVDs long.

   Depositor

   Andrew Scharenberg

   Article

   Boissel et al Nucleic Acids Res. 2013 Nov 26.

   Type

   Empty backbone

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUSC10

    Plasmid

    #50430

    Purpose

    Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 10.5 RVDs long.

    Depositor

    Andrew Scharenberg

    Article

    Boissel et al Nucleic Acids Res. 2013 Nov 26.

    Type

    Empty backbone

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPCR-Script-mLIP

    Plasmid

    #49194

    Purpose

    initial cloning vector for mLIP fragment of C/EBPbeta

    Depositor

    Jed Friedman

    Insert

    C/EBPbeta (LIP fragment) (Cebpb Mouse)
    

    Use

    Cloning vector

    Expression

    Bacterial

    Mutation

    only the LIP fragment

    Promoter

    lac

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pBS ZfHoxa9a

    Plasmid

    #27518

    Depositor

    Victoria Prince

    Article

    Prince et al Development. 1998 Feb . 125(3):407-20.

    Insert

    Hoxa9a (hoxa9a Zebrafish)
    

    Use

    Cloning vector

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

13. ARL5:H14-K177 (2H16)

    Plasmid

    #25526

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ARL5:H14-K177 (ARL5A Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids H14-K177

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pAdTrack-CMV

    Plasmid

    #16405

    Purpose

    Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-IRES-GFP II (pMIG II)

    Plasmid

    #52107

    Purpose

    a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. p5E-elavl3

    Plasmid

    #82400

    Purpose

    Gateway-compatible zebrafish elavl3 promoter

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    elavl3 promoter
    

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-eGFP

    Plasmid

    #36083

    Purpose

    3rd generation lentiviral transfer plasmid

    Depositor

    Pantelis Tsoulfas

    Article

    Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC18-mini-Tn7T-Gm-lux

    Plasmid

    #64963

    Purpose

    mini-Tn7 luxCDABE transcriptional fusion vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    lux operon
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. p663-UBC-TurboID-V5-BRSK2_IDG-K

    Plasmid

    #135272

    Purpose

    Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-BRSK2 (BRSK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAWP89

    Plasmid

    #61264

    Purpose

    IncP-based replicating vector expressing dTomato under tac promoter.

    Depositor

    Mary Lidstrom

    Article

    Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

    Insert

    Ptac-dTomato
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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