We narrowed to 5,362 results for: crispr cas9 grna plasmid

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pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA1

Plasmid

#220117

Purpose

Expresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d start codon site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSauriCas9

Use

AAV

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA6

Plasmid

#220118

Purpose

Expresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d intron6 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSauriCas9

Use

AAV

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA7

Plasmid

#220119

Purpose

Expresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSauriCas9

Use

AAV

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA1

Plasmid

#220120

Purpose

Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d start codon site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauriCas9

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA6

Plasmid

#220122

Purpose

Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron6 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauriCas9

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA7

Plasmid

#220124

Purpose

Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauriCas9

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-HA-TadA8e-Sauri-U6-Camk2d sgRNA7

Plasmid

#220127

Purpose

Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter, and add an HA tag fused to TadA

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauriCas9

Use

AAV

Tags

HA tag

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold

Plasmid

#226935

Purpose

Encoding SaKKHABE8e and sgRNA cassette on a single AAV

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSaKKHCas9

Use

AAV

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pX552-DIO-NLS-mRuby3(with gRNA scaffold)

Plasmid

#199583

Purpose

Expresses NLS-mRuby3 in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

NLS-mRuby3

Use

AAV, CRISPR, and Cre/Lox

Expression

Mammalian

Promoter

U6 and EF1a

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKLF6.1.0-gDNA

Plasmid

#132435

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

KLF6 (KLF6 Human)

Use

CRISPR

Available Since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pKLF10.1.0-gDNA

Plasmid

#132433

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

KLF10 (KLF10 Human)

Use

CRISPR

Available Since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pJOG253

Plasmid

#80585

Purpose

recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)

Plasmid

#199582

Purpose

Expresses Chronos-eGFP in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Chronos eGFP

Use

AAV, CRISPR, and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

EF1a intron A

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

Plasmid

#177360

Purpose

AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Inserts

tdTomato
Chimeric guide for SaCas9

Use

AAV

Tags

P2A and a tetracycline-controlled transactivator …

Expression

Mammalian

Promoter

Synapsine promoter and U6 promoter

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

Plasmid

#177365

Purpose

AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Inserts

tdTomato
Chimeric guide for SaCas9

Use

AAV

Tags

P2A and a tetracycline-controlled transactivator …

Expression

Mammalian

Promoter

CMV and U6 promoter

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-inteinC-aa713 SpG C-U6- sgRNA scaffold

Plasmid

#208111

Purpose

Expresses SpG cas9C by the constitutive CASI promoter and sgRNA scaffold by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

SpG C, U6, scaffold

Use

AAV

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-InteinC-NG C aa714-1368-U6-Lmna sgRNA1

Plasmid

#206974

Purpose

Expresses NG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

NG aa714-1368, U6, Lmna sgRNA1

Use

AAV

Promoter

U6

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

Plasmid

#160570

Purpose

A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA:scaffold tetraloop MS2 aptamer SAM

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-PCNT

Plasmid

#227284

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of PCNT (PCNT Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-RAB7A

Plasmid

#227297

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of RAB7A (RAB7A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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