We narrowed to 29,671 results for: des.2

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SARS-COV2-NSP1-HA-BASU

Plasmid

#154071

Purpose

Express SARS-CoV-2 NSP1 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.2 (20-362)

Plasmid

#177845

Purpose

Bacterial Expression of SnRK2.2

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2 (SNRK2.2 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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An HA CAAX pMT2

Plasmid

#206116

Purpose

expression of Cav2.1 N-terminus (amino acids 1-100) with a C-terminal CAAX (last 10aa of H.Ras) motif and a HA tag

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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An CAAX pMT2

Plasmid

#206118

Purpose

expression of Cav2.1 N-terminus (amino acids 1-100) with C-terminal CAAX (last 10 aa of H.Ras) motif

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pHDT2:GUS

Plasmid

#108444

Purpose

Arabidopsis transformation, expresses HDT2 promoter/GUS fusion to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT2 promoter (HD2B Mustard Weed)

Tags

GUS

Expression

Plant

Promoter

HDT2

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHDT2:HDT2-GFP

Plasmid

#108566

Purpose

Arabidopsis transformation, expresses HDT2/GFP fusion with HDT2 promoter to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT2 (HD2B Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

HDT2

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_CUS1F03

Plasmid

#71071

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Su(var)205 (Su(var)205 Fly)

Use

Gateway entry vector

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-GFP-IMP2-2_antisense-control

Plasmid

#42174

Depositor

Alexandra Kiemer

Article

Kessler et al Am J Physiol Gastrointest Liver Physiol.

Insert

Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)

Tags

GFP

Expression

Mammalian

Promoter

T7

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
pEZYeGFP-SARS-CoV-2E GGG

Plasmid

#224582

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 with a mutated PBM in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E GGG (E SARS-CoV-2)

Tags

GFP

Expression

Mammalian

Mutation

PBM (LLV) mutated to GGG

Promoter

CMV

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

Plasmid

#157702

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

Plasmid

#157701

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEZYeGFP-SARS-CoV-2E stop

Plasmid

#224581

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 lacking its PBM in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E stop (E SARS-CoV-2)

Tags

GFP

Expression

Mammalian

Mutation

stop codon before PBM

Promoter

CMV

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Bn R52A, R54A pMT2

Plasmid

#206087

Purpose

Cav2.2 N-terminus (amino acids 1-95) with R52A and R54A mutations that prevent dominant-negative suppression of Cav2.2 currents by truncated Cav2.2

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

R52A, R54A

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_535-775 USP7

Plasmid

#131247

Purpose

Mammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pIA1385

Plasmid

#166862

Purpose

Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGTM_YR375_SUMO_Protease_001

Plasmid

#190063

Purpose

Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

Depositor

Gaetano Montelione

Article

Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

Insert

ULP1_YEAST (ULP1 Budding Yeast)

Tags

8X His-MBP (Maltose Binding Protein)

Expression

Bacterial

Mutation

Construct contains residues 347 – 621

Promoter

T7 lac

Available Since

Jan. 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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helicase cDNA

Plasmid

#39552

Depositor

Norbert Perrimon

Article

Eberl et al Genetics. 1997 Jul;146(3):951-63.

Insert

helicase (Hel25E Fly)

Available Since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only
pCI-MOR1-1

Plasmid

#22327

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR1-1 (Olfr64 Mouse)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pFM038

Plasmid

#40141

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

R183-5A + 7SA; base pairs 6-523 of par-2 were re…

Promoter

pie-1

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM041

Plasmid

#40144

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (S241A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

S241A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only