We narrowed to 6,391 results for: siae
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Plasmid#84632PurposeUnimolecular, FRET-based JNK activity reporter; cytosol targetedDepositorInsertECFP-FHA1-JNKsubDD-Citrine-NES
Tags6xHis, Citrine, ECFP, Nuclear export signal (NES)…ExpressionMammalianPromoterCMVAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2803
Plasmid#63646PurposepCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2899(X-2 MarkerFree)
Plasmid#73271PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K9R
Plasmid#84527PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidMutationK(43,61,69,117,240,246,527,582,651)RPromoternoneAvailable SinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV114
Plasmid#63181PurposepRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17
Plasmid#121386PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastPromoterADH1 promoterAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean-WW-NES
Plasmid#84629PurposeEncodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertCerulean-WW-NES
Tags6xHis, Cerulean, Nuclear export signal (NES), T7 …ExpressionMammalianPromoterCMVAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEK20
Plasmid#210582PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdGAPDHpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdGAPDHproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS415-Cas9-VPR
Plasmid#163971PurposeTrifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeastDepositorInsertCas9-VPR
UseSynthetic BiologyExpressionYeastAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB2804
Plasmid#63644PurposepCfB2804 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHES873
Plasmid#87945PurposeExpresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertpGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Human, Budding Yeast)
ExpressionYeastPromoterGAL1Available SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-GFPIvy-SpHis5
Plasmid#60783PurposeYeast C-terminal tagging vector, which creates gene fusions with GFPIvy using SpHis5 selectionDepositorInsertGFPIvy
UseYeast integratingMutationS30R Y39N T65C Q69A S72A F99S N105T Y145F M153T V…Available SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-ODC1
Plasmid#133256PurposeExpress FLAG-tagged ODC1 in mammalian cellsDepositorInsertODC1 (ODC1 Budding Yeast)
UseRetroviralTags3xFLAGExpressionMammalianMutationcodon-optimized for expression in human cellsAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG4-E6s-luc
Plasmid#46324DepositorInsert5xGal4
UseLuciferaseTagsE boxes and luciferaseExpressionMammalianMutationfive copies of a multimerized GAL4 (5×GAL4) site …Available SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK2
Plasmid#118092PurposeExpresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)
Plasmid#122067PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
TagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAST mito roGFP2-Orp1
Plasmid#64997Purposefor expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophilaDepositorInsertOrp1 (HYR1 Budding Yeast)
TagsroGFP2ExpressionInsectMutationmitochondrial targeting sequenceAvailable SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-HJURP-CBP
Plasmid#30393DepositorAvailable SinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGST-TEV-yVms1(1-417,delta(38-69))
Plasmid#128739PurposeBacterial overexpression and crystallizationDepositorInsertVms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)
TagsGSTExpressionBacterialMutationresidues 1-417 with deletion of 38-69PromoterT7Available SinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
IpO
Plasmid#48233PurposeBase vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.DepositorInsertsURA3 3' fragment
URA3 5' fragment
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterURA3 promoter and noneAvailable SinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only