We narrowed to 162,717 results for: addgene

1. pW401-lenti-sg1-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #189950

   Purpose

   Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg1-Itgb1) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

   Insert

   NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pW402-lenti-sg2-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #189951

   Purpose

   Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg2-Itgb1) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

   Insert

   NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pW403-lenti-sg3-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #189952

   Purpose

   Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg3-Itgb1) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

   Insert

   NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCL.037

   Plasmid

   #184966

   Purpose

   Express -Eco1 dead RT and ncRNA in yeasst

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Human codon optimized RT, YXDD catalytic region m…

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCL.71

   Plasmid

   #184983

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker1

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker1-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCL.112

   Plasmid

   #184981

   Purpose

   Express -Eco1 FAA1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   FAA1 donor P233stop

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCL.002

   Plasmid

   #184972

   Purpose

   Express -Eco1 ADE2 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ADE2 donor P272stop

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCL.110

   Plasmid

   #184979

   Purpose

   Express -Eco1 TRP2 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   TRP2 donor E64stop

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKLV2-EF1a-GFP ArgSen delta Degradation Domain

   Plasmid

   #184692

   Purpose

   Lentiviral transfer plasmid for expression of GFP ArgSen delta Degradation Domain

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   GFP ArgSen delta Degradation Domain
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKLV2-EF1a-GFP ArgSen delta Disordered Region

    Plasmid

    #184693

    Purpose

    Lentiviral transfer plasmid for expression of GFP ArgSen delta Disordered Region

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP ArgSen delta Disordered Region
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKLV2-EF1a-GFP ArgSen delta Rad23b UbL

    Plasmid

    #184694

    Purpose

    Lentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbL

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP ArgSen delta Rad23b UbL
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-EF1a-GFP ArgSen delta Degradation Domain

    Plasmid

    #184701

    Purpose

    Lentiviral transfer plasmid for expression of GFP ArgSen delta Degradation Domain

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP ArgSen delta Degradation Domain
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-EF1a-GFP ArgSen delta Disordered Region

    Plasmid

    #184702

    Purpose

    Lentiviral transfer plasmid for expression of GFP ArgSen delta Disordered Region

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP ArgSen delta Disordered Region
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. XLone-BSD NFIL3

    Plasmid

    #179512

    Purpose

    Tunable and temporal expression control of NFIL3

    Depositor

    Xiaoping Bao

    Article

    Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

    Insert

    NFIL3 (NFIL3 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS Promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19_HR-BirA-FLAG_Ago2

    Plasmid

    #170918

    Purpose

    Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    BirA-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    lac

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19_HR-BirA-FLAG_Ago1

    Plasmid

    #170919

    Purpose

    Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    BirA-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    lac

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPLN-hFluc-WT-KDEL

    Plasmid

    #177715

    Purpose

    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    KDEL and Prolactin Signal Sequence

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pStr-KDEL_APV-SBP-Dendra2-ER-5

    Plasmid

    #170679

    Purpose

    Expresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Inserts

    APV-SBP-Dendra2-ER-5
    streptavidin-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and T7

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #170812

    Purpose

    Lentiviral vector to co-express an sasgRNA with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-null (BPK1077)

    Plasmid

    #171128

    Purpose

    Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    pCMV and T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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