We narrowed to 11,999 results for: SHA
-
Plasmid#170386PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Cre-inducible expression of nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVPromoterhuman SynapsinAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.3.hSyn.flex.H2B.RFP
Plasmid#170374PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Cre-inducible expression of nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVPromoterhuman SynapsinAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.13.hSyn.flex.H2B.RFP
Plasmid#170376PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Cre-inducible expression of nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVPromoterhuman SynapsinAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB’ CsoS2 ΔNTD
Plasmid#162705PurposeCarboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2DepositorInsertpHnCB10 derived carboxysome operon, prk
ExpressionBacterialMutationDeletion of residues 2-256 of CsoS2, Y131DPromoterPLtet0-1 promoterAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(K206A/K244A)
Plasmid#177135PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A) with a TEV protease site located between the GST tag and PolB(K206A/K244A)DepositorInsertPolB
TagsGSTExpressionBacterialPromoterTacAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42 -SSRP1
Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
R9-FS1
Plasmid#125208PurposeExpressing human Rad9b frameshift mutation found in a neural tube defect patientDepositorAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
R9-FS2
Plasmid#125209PurposeExpressing human Rad9b frameshift mutation found in a neural tube defect patientDepositorAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 delta787-843
Plasmid#166821PurposeGateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Ub-NLRP1 S1213 P1214R
Plasmid#166830PurposepDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 T755N
Plasmid#166820PurposeGateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 R726W
Plasmid#166819PurposeGateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C R459E
Plasmid#164024PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C R464E
Plasmid#164025PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C E485R
Plasmid#164026PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C E490R
Plasmid#164027PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C R495E
Plasmid#164028PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C D511K
Plasmid#164029PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C Y527A
Plasmid#164030PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim Actin MCH Puro (CAMP)
Plasmid#104446PurposeExpresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…PromoteractinAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C NLRP1 CARD G1467C
Plasmid#164009PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD G1467CDepositorAvailable SinceFeb. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-dt/CBA-EGFP-NLS
Plasmid#163918PurposeContains bidirectional reporter genes dtomato and nuclear-targeting EGFP.DepositorInsertdtomato and EGFP
UseLentiviralExpressionMammalianMutationAdded nuclear localization signal to EGFPAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCM’ CbbQ-
Plasmid#162711PurposeSecond CCM operon with inactive CbbQ (K46A, E107Q) rubisco activaseDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationInactivation of CbbQ subunit of CbbOQ rubisco act…PromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCM’ ΔacRAF
Plasmid#162710PurposeSecond CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAFDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationpCCM' with a deletion of putative rubisco ch…PromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTripZ-B2
Plasmid#163924PurposeFor making a cell line that expresses doxycyline-inducible RNAi suppressor protein B2 for increasing titer of lentivirus with bidirectional promoters during virus production.DepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisA-HOXC6-2
Plasmid#110365PurposeMammalian expression of HisA-tagged HOXC6-2DepositorAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-Unstructured-EIF3B-465-552
Plasmid#136029PurposeEIF3B (465-552) 3'UTR with the Artificial Unstructured 3'UTR fused upstream inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-EIF3B-88nt-Delta-Hairpin
Plasmid#136030PurposeEIF3B (465-552) 3'UTR with the main hairpin deleted inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-EIF3B-88nt-1st-Hairpin
Plasmid#136031PurposeEIF3B (465-552) 3'UTR with only the main hairpin inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-EIF3B-88nt-Disrupted-Hairpin
Plasmid#136032PurposeEIF3B (465-552) 3'UTR with the main hairpin mutated inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only