We narrowed to 19,038 results for: Cre

1. p28BIOHTEV-LIC: nsp10_SARS-CoV-2

   Plasmid

   #167847

   Purpose

   Bacterial expression of the SARS-CoV-2 protein nsp10

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp10
   

   Use

   Tags

   6xHis and AviTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 1, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. CPH3567 (pET151_Spt6 1247-1451 R1282H)

   Plasmid

   #91816

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed arginine 1282 to histidine; deleted resid…

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CPH3495 (pET151_Spt6 1247-1451 K1435A)

   Plasmid

   #91817

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1435 to alanine; deleted residues …

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RAP2A-p2A-E2Crimson

   Plasmid

   #83940

   Purpose

   Lentiviral vector expressing RAP2A-p2A-E2Crimson

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   p2A-E2Crimson

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SLC35B2-p2A-E2Crimson

   Plasmid

   #83941

   Purpose

   Lentiviral vector expressing SLC35B2-p2A-E2Crimson

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   SLC35B2 (SLC35B2 Human)
   

   Use

   Lentiviral

   Tags

   p2A-E2Crimson

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-sgALCAM

   Plasmid

   #83929

   Purpose

   Lentiviral vector expressing an sgRNA targeting ALCAM. NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgALCAM
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-sgSLC35B2

   Plasmid

   #83932

   Purpose

   Lentiviral vector expressing an sgRNA targeting SLC35B2 NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgSLC35B2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-sgTPST2

   Plasmid

   #83933

   Purpose

   Lentiviral vector expressing an sgRNA targeting TPST2 NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgTPST2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV_eA3A T31A-nCas9

   Plasmid

   #163538

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)eA3A T31A

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV_UdgX-EE-UdgX-nCas9

    Plasmid

    #163564

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 1010-AIDx-CP

    Plasmid

    #135340

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1010 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1010 nSpyCas9 -hAIDx
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 1058-AIDx-CP

    Plasmid

    #135342

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1058 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1058 nSpyCas9-hAIDx
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AIDx-ID-208

    Plasmid

    #135345

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 208 of nSpyCas9

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-208
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AIDx-ID-468

    Plasmid

    #135346

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 468 of nSpyCas9

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-468
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-X1-Neo-DDRGK1-K227R-HA

    Plasmid

    #139859

    Purpose

    Lentiviral expression of human DDRGK1-K227R-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    K227R

    Promoter

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-X1-Neo-DDRGK1-K121R-HA

    Plasmid

    #139852

    Purpose

    Lentiviral expression of the cDNA indicated

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    K121R

    Promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLB(N)CX-FLAG-CH3 Del33

    Plasmid

    #133724

    Purpose

    Retroviral vector with CMV promoter driving mutant form of CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.

    Depositor

    James A. DeCaprio

    Article

    Borger et al J Virol. 2006 May . 80(9):4292-303.

    Insert

    EP300 (EP300 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    partial (not complete) p300 fragment, AT and CH3 …

    Promoter

    CMV

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLB(N)CX AT-CH3-FLAG-HA WY

    Plasmid

    #133725

    Purpose

    Retroviral vector with CMV promoter driving mutant form of acetyltransferase and CH3 domains fragment of human p300 (EP300) (WY1466-1467AS in AT domain).

    Depositor

    James A. DeCaprio

    Article

    Borger et al J Virol. 2006 May . 80(9):4292-303.

    Insert

    EP300 (EP300 Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mutation

    partial (not complete) p300 fragment, AT and CH3 …

    Promoter

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-X1-Neo-DDRGK1-K116R-HA

    Plasmid

    #139850

    Purpose

    Lentiviral expression of human DDRGK1-K116R-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    K116R

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-X1-Neo-DDRGK1-K120R-HA

    Plasmid

    #139851

    Purpose

    Lentiviral expression of human DDRGK1-K120R-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    K120R

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-X1-Neo-DDRGK1-K124R-HA

    Plasmid

    #139853

    Purpose

    Lentiviral expression of human DDRGK1-K124R-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    K124R

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-X1-Neo-DDRGK1-K128R-HA

    Plasmid

    #139854

    Purpose

    Lentiviral expression of human DDRGK1-K128R-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    K128R

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-X1-Neo-DDRGK1-K146R-HA

    Plasmid

    #139855

    Purpose

    Lentiviral expression of human DDRGK1-K146R-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    K146R

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-X1-Neo-DDRGK1-K176R-HA

    Plasmid

    #139856

    Purpose

    Lentiviral expression of human DDRGK1-K176R-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    K176R

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-X1-Neo-DDRGK1-K193R-HA

    Plasmid

    #139857

    Purpose

    Lentiviral expression of the cDNA indicated

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    K193R

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-X1-Neo-DDRGK1-K224R-HA

    Plasmid

    #139858

    Purpose

    Lentiviral expression of human DDRGK1-K224R-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    K224R

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126753

    Purpose

    Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. actc1b-mKate2-jph1a

    Plasmid

    #109497

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to junctophilin 1a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    jph1a (jph1a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Please see Depositor Comments

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p3E-ARF1-DN

    Plasmid

    #109590

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative ARF1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF1 (arf1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p3E-ARF6-DN

    Plasmid

    #109592

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative ARF6

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF6 (arf6a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p3E-SERCA1

    Plasmid

    #109552

    Purpose

    Multisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    sarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. actc1b-mKate2-ARF1

    Plasmid

    #109535

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to ARF1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF1 (arf1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. actc1b-Cav3-mKate2

    Plasmid

    #109518

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to Caveolin3

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Cav3 (cav3 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. actc1b-mKate2-Dnm2a-DN

    Plasmid

    #109520

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to dominant negative Dynamin2a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Dnm2a (dnm2a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    DN

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNCST-AvicFP3

    Plasmid

    #129506

    Purpose

    Expresses AvicFP3 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GS2.1/EC

    Plasmid

    #132568

    Purpose

    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    egg cell promoter, Cas9-mApple, pds3 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    A. thaliana egg cell promoter

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pbIG-Xph20-Stg

    Plasmid

    #133035

    Purpose

    Expresses Xph20-Stg in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20-Stg
    

    Use

    Tags

    AviTag, His10, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pbIG-Xph18-ETWV

    Plasmid

    #133036

    Purpose

    Expresses Xph18-ETWV in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph18-ETWV
    

    Use

    Tags

    AviTag, His10, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pbIG-Xph18-Stg

    Plasmid

    #133037

    Purpose

    Expresses Xph18-Stg in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph18-Stg
    

    Use

    Tags

    AviTag, His10, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pbIG-Xph15-ETWV

    Plasmid

    #133038

    Purpose

    Expresses Xph15-ETWV in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph15-ETWV
    

    Use

    Tags

    AviTag, His10, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge