We narrowed to 18,127 results for: Mut

1. pJK325

   Plasmid

   #71556

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes alanine-367 to aspartate

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMES140

   Plasmid

   #71849

   Purpose

   Complementation of NME1 gene disruption or mutation in yeast

   Depositor

   David Clayton, Mark Schmitt

   Article

   Schmitt et al Mol Cell Biol. 1993 Dec;13(12):7935-41.

   Insert

   NME1 (NME1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   NME1

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJexpress414_OmpA171_R4cons_P121F

   Plasmid

   #64301

   Purpose

   Conservative version of OmpA Redesign 4 with P121F mutation

   Depositor

   Timothy Whitehead

   Article

   Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

   Insert

   OmpA_R4cons_P121F
   

   Use

   Synthetic Biology

   Tags

   6His and FlAsH

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pJexpress414_OmpA171_R4cons_G99I

   Plasmid

   #64300

   Purpose

   Conservative version of OmpA Redesign 4 with G99I mutation

   Depositor

   Timothy Whitehead

   Article

   Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

   Insert

   OmpA_R4cons_G99I
   

   Use

   Synthetic Biology

   Tags

   6His and FlAsH

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pJexpress414_OmpA171_R4cons_G125L

   Plasmid

   #64298

   Purpose

   Conservative version of OmpA Redesign 4 with G125L mutation

   Depositor

   Timothy Whitehead

   Article

   Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

   Insert

   OmpA_R4cons_G125L
   

   Use

   Synthetic Biology

   Tags

   6His and FlAsH

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pICSL80012

   Plasmid

   #50331

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, YFP variant of GFP (A. victoria)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pICH71292

   Plasmid

   #50280

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter and 5'UTR, RbcS2 (S. lycopersicum)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pICH71311

   Plasmid

   #50282

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter and 5'UTR, RbcS3A (S. lycopersicum)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pICSL50012

   Plasmid

   #50311

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   C terminal V5 tag (Simian Virus 5)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pICSL50013

    Plasmid

    #50312

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal T7 tag (bacteriophage T7 gene 10)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICH71301

    Plasmid

    #50281

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter and 5'UTR, RbcS2 (S. lycopersicum)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICH37431

    Plasmid

    #50306

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    signal peptide, RAmy3A (O. sativa)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pICH45089

    Plasmid

    #50254

    Depositor

    Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter (double), 35s (Cauliflower Mosaic Virus)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pICH42760

    Plasmid

    #50257

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter, spm (Z. mays)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDL128

    Plasmid

    #43912

    Depositor

    Stephen Elledge

    Article

    Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

    Insert

    DIF1 (DIF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    SML domain mutated PPMLINQRT->AAAAAAAA, S59G, …

    Promoter

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pDL127

    Plasmid

    #43911

    Depositor

    Stephen Elledge

    Article

    Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

    Insert

    DIF1 (DIF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    HUG domain mutated VGMRIRQ->AAAAAAA, S59, Q66H

    Promoter

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pEXP5-NT-AlleyCat

    Plasmid

    #41715

    Depositor

    Ivan Korendovych, William DeGrado

    Article

    Korendovych et al Proc Natl Acad Sci U S A. 2011 Apr 26;10

    Insert

    mutated C-terminal portion of calmodulin gene (M76-K148)
    

    Use

    Tags

    Polyhistidine (6×His) region, TEV recognition sit…

    Expression

    Bacterial

    Mutation

    F92E

    Promoter

    T7

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CJ26 (miR-1003 3'ssAG->GA)

    Plasmid

    #17765

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    dme-miR-1003 minigene 3'ssAG->GA
    

    Use

    Drosophila metallothionein gene promoter drives e…

    Tags

    Expression

    Insect

    Mutation

    splice site mutation

    Promoter

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

19. C205

    Plasmid

    #174359

    Purpose

    For expressing siRNA-resistant frog ePAB in human cell lines via transfection

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    ePAB
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Multiple silent mutations introduced to be resist…

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pABE-dGFP

    Plasmid

    #226863

    Purpose

    Constitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by A111V mutation, that can be reactivated by active adenine base editing

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A111V

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pABE-dGFP-gRNA

    Plasmid

    #226864

    Purpose

    Human gRNA expression vector targeting the A111V mutation of non-fluorescent EGFP in plasmid pABE-dGFP; for use with ABEs

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    Sp-gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDB327

    Plasmid

    #113671

    Purpose

    expresses Ulp1_R3 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R3
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TEVSH

    Plasmid

    #125194

    Purpose

    His-tagged TEV protease with solubility increasing mutations

    Depositor

    Helena Berglund

    Article

    van den Berg et al J Biotechnol. 2006 Feb 10;121(3):291-8.

    Insert

    TEV_SH
    

    Use

    Tags

    V5 His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSAM_Ec

    Plasmid

    #102939

    Purpose

    Transposon mutagenesis and Tn-seq

    Depositor

    Matthew Mulvey

    Article

    Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

    Insert

    mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pICSL50004

    Plasmid

    #50316

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, mCherry variant of RFP (Discosoma sp.)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pICSL30003

    Plasmid

    #50304

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, no stop codon, mCherry variant of RFP (Discosoma sp.)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. K-SPOTIT1.0

    Plasmid

    #171215

    Purpose

    Expresses a kappa-opioid receptor-based circular permuted green fluorescent protein sensor

    Depositor

    Wenjing Wang

    Article

    Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

    Insert

    K-SPOTIT1.0
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 EYFP H148Q/I152L

    Plasmid

    #25872

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q/I152L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q and I152L m…

    Promoter

    Available Since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMBP-MS2

    Plasmid

    #65104

    Purpose

    can be used to isolate RNA binding complexes

    Depositor

    Josep Vilardell

    Article

    Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

    Insert

    MS2
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    double mutation (V75Q and A81G) that prevents oli…

    Promoter

    tac

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

    Plasmid

    #159905

    Purpose

    Mutagenesis of Slc32a1

    Depositor

    Larry Zweifel

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSAM_Cam

    Plasmid

    #91570

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

32. pU6_N7-sgRNA2

    Plasmid

    #190664

    Purpose

    Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    N7 sgRNA2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-CMV-FLEX-SaCas9-U6-sgDrd2

    Plasmid

    #159918

    Purpose

    Mutagenesis of Drd2

    Depositor

    Larry Zweifel

    Insert

    Drd2 (Drd2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEB1-mVenus NB

    Plasmid

    #103986

    Purpose

    Plasmid encoding mVenus NB

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mVenus NB
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMF19

    Plasmid

    #113662

    Purpose

    Containing the wbbL (rhamnosyltransferase) gene cloned for mutant complementation

    Depositor

    Miguel Valvano

    Article

    Feldman et al J Biol Chem. 1999 Dec 3;274(49):35129-38

    Insert

    wbbL
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Ptac

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pICH41421

    Plasmid

    #50339

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEB1-SCFP3A

    Plasmid

    #103970

    Purpose

    Plasmid encoding SCFP3A

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    SCFP3A
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1 Hygro EYFP H148Q/I152L

    Plasmid

    #25874

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q/I152L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q and I152L m…

    Promoter

    Available Since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-MS2-HA-HshnRNPA2-B1_Z

    Plasmid

    #148113

    Purpose

    Mammalian Expression of HshnRNPA2-B1

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPA2-B1 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    splicing variant of hnRNPA2 variant B1. Exon 6 an…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSFFV_sfCherry2_TagBFP

    Plasmid

    #83031

    Purpose

    Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry+full length_TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    3 mutations in sfCherry full length: E118Q, T128I…

    Promoter

    SFFV

    Available Since

    Jan. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge