164,577 results

1. peGFP C3-SPG20-PAAA

   Plasmid

   #218581

   Purpose

   Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   changed Proline 172 to Alanine and Tyrosine 174 t…

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. peGFP C1-SPG20 SC domain

   Plasmid

   #218580

   Purpose

   Expresses GFP-tagged SC domain of human SPG20 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-417

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. C1(1-29)-TurboID-V5_pCDNA3

   Plasmid

   #107173

   Purpose

   expresses V5-tagged TurboID on the mammalian ER membrane

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3-HA-human MYCN

   Plasmid

   #74163

   Purpose

   Mammalian expression of human MycN

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   MycN (MYCN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_DYNLL1_Dynein-light

   Plasmid

   #110006

   Purpose

   Protein expression and purification of DYNLL1_Dynein-light

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   DYNLL1_Dynein-light (DYNLL1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEN_TT 3xFLAG-TWIST2

   Plasmid

   #197935

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged TWIST2

   Depositor

   Kevin Janes

   Article

   Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

   Insert

   TWIST2 (TWIST2 Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Rosa26-LSL-PE2 Targeting Vector

   Plasmid

   #196038

   Purpose

   The targeting construct contains a CAGG promoter, FRT site, loxP–stop-loxP, the PE2 enzyme, P2A peptide, an mNeonGreen reporter, WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA.

   Depositor

   Tyler Jacks

   Article

   Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

   Insert

   Cas9-MMLV-RT
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-ZIKVA-GFP-puro

   Plasmid

   #140090

   Purpose

   Fluorescence activatable reporter of ZIKV NS2B-NS3 protease activity

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

   Insert

   ZIKVA-GFP
   

   Use

   Lentiviral; Low expression

   Tags

   6XHis tag and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSPAsp-hp

   Plasmid

   #48122

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

   Insert

   predicted optimal signal peptide for Bacillus megaterium protein secretion
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSG5-BZLF1

    Plasmid

    #72637

    Purpose

    Mammalian expression vector for Epstein-Barr virus Zta immediate-early transcriptional regulator

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BZLF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q163R

    Promoter

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pMSCV-IRES-mCherry FP

    Plasmid

    #52114

    Purpose

    a bicistronic IRES-mCherry containing retroviral vector

    Depositor

    Dario Vignali

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-CuR-hSOD1

    Plasmid

    #232477

    Purpose

    The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-CuO

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-DENV2A-GFP-puro

    Plasmid

    #140089

    Purpose

    Fluorescence activatable reporter of DENV-2 NS2B-NS3 protease activity

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

    Insert

    DENV2A-GFP
    

    Use

    Lentiviral; Low expression

    Tags

    6XHis tag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #126216

    Purpose

    Labeling of excitatory synapses (green fluorescence) in cre-expressing neurons

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95.FingR-eGFP-CCR5TC
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. SSTR5-Tango

    Plasmid

    #66506

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    SSTR5 (SSTR5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. C14orf169

    Plasmid

    #39114

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    C14orf169 (C14orf169 Human)
    

    Use

    Tags

    Tev His Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. mIFP-VASP-5

    Plasmid

    #56241

    Purpose

    Localization: Focal Adhesions, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    VASP (Vasp Mouse)
    

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pMRX-IP-EGFP-mouse Plaat3 (N157stop)

    Plasmid

    #168771

    Purpose

    Expresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Hrasls3 (Plaat3 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMV-LEU2

    Plasmid

    #65334

    Purpose

    standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    LEU2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNCS mCherry

    Plasmid

    #91769

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Cherry
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    See Depositor Comments

    Promoter

    T7

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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