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pDONR223-IKBKE

Plasmid

#23536

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

IKBKE (IKBKE Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCDF1-p18INK4c

Plasmid

#25650

Depositor

Todd Waldman

Article

Solomon et al Cancer Res. 2008 Apr 15. 68(8):2564-9.

Insert

p18INK4c (CDKN2C Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago2K533AK570A

Plasmid

#14104

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Lys533 to Ala, Lys570 to Alanine

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_V5-NEUROD1

Plasmid

#215031

Purpose

Mammalian expression of human NEUROD1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

neuronal differentiation 1 (NEUROD1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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SERPINH1

Plasmid

#155999

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SERPINH1 (SERPINH1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pXD023 - pLKO-EF1a-Puro-P2A-FLuc-WPRE

Plasmid

#192203

Purpose

Lenti-PL(Puro-Luciferase)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-PL(Puro-Luciferase)

Use

Lentiviral; Mammalian expression

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-NES

Plasmid

#171006

Purpose

HiLITR protease with C-terminal NNES (Cytoplasmic)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-NES

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-GRK2RH-Nluc

Plasmid

#158605

Purpose

Plasmid expressing GRK2(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET22b-mEB1-eGFP-6xHis

Plasmid

#130981

Purpose

Bacterial expression of mouse EB1-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB1-GFP-6xHis (Mapre1 Mouse)

Tags

eGFP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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EMR2_pLX307

Plasmid

#98332

Purpose

Lentiviral expression of EMR2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

EMR2 (ADGRE2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

Plasmid

#90473

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Insert

TagGFP2-TEV-GGG-ELP(120nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pUB-spHRed

Plasmid

#84734

Purpose

Expresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stoma

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

pHRed

Tags

RbcS targeting sequence

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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CDK4-luciferase full length (2135)

Plasmid

#86656

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

2120 base pairs including up to coding nucleotide…

Promoter

CDK4

Available Since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

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mTFP1-TOMM20-N-10

Plasmid

#55511

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

TOMM20 (TOMM20 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
p6898 PHAGE-P CMVT N-HA-hE6AP I C820A

Plasmid

#37602

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP I C820A (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C820A

Promoter

CMV tet regulatable

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pBSFI-p110γ

Plasmid

#34892

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2006 Jan 31;10

Insert

p110γ (PIK3CG Human)

Use

Adaptor plasmid

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only
Dynamin-I-K44A-6His/Flag

Plasmid

#32696

Depositor

Robert Lefkowitz

Article

Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

Insert

Dynamin I (Dnm1 Rat)

Tags

Flag and His6

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pJAT10

Plasmid

#204293

Purpose

For marking CRISPR HDR integrant with DsRed expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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bdSUMO-TAT-GFP-HiBit

Plasmid

#170991

Purpose

For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-TAT-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-O-RUNX3-T2A-PuroR

Plasmid

#162349

Purpose

Lentiviral expression of RUNX3 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

RUNX3 (RUNX3 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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