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We narrowed to 18,247 results for: multi

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Showing: 5261 - 5280 of 18247 results

  1. pJW31

    Plasmid
    #136423
    Purpose
    Contains genes of Type I-C CRISPR-Cas system for inducible expression and chromosomal integration at attTn7
    Depositor
    Joseph Bondy-Denomy
    Publication
    Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas5
    Cas8
    Cas7
    Cas3
    Cas3
    Use
    CRISPR
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    136423-plasmid-map-sequence-id-270560

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  5. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  8. pMM643

    Plasmid
    #112533
    Purpose
    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR
    Depositor
    Timothy Lu
    Publication
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    ChuA
    luxCDABE
    Expression
    Bacterial
    Mutation
    A17P
    Promoter
    J23107, PL(HrtO), and ProD
    Available since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    112533-plasmid-map-sequence-id-216001

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  9. pCR1054

    Plasmid
    #71822
    Purpose
    Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS
    Depositor
    Jacob Corn
    Publication
    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
    Use
    CRISPR
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71822-plasmid-map-sequence-id-131019

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  10. pU1/Sm/3' Box_(GLuc)_TOP1

    Plasmid
    #68437
    Purpose
    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOP1 construct
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U1
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    68437-plasmid-map-sequence-id-124681

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  12. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

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  13. pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

    Plasmid
    #78251
    Purpose
    Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    -3mnx1 (mnx1 Zebrafish)
    -loxP-mTagBFP2-stop-
    mCherry-NS-MCS
    Use
    Zebrafish motor neuron inducible expression
    Promoter
    -3mnx1
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    78251-plasmid-map-sequence-id-149769

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  15. EKARet-nuc

    Plasmid
    #96896
    Purpose
    FLIM sensor for ERK activation in nucleus
    Depositor
    Ryohei Yasuda
    Publication
    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLIM Sensor for ERK Activity in Nucleus
    Tags
    EGFP and sREAChet
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    96896-plasmid-map-sequence-id-182390

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  16. p3E-mTagBFP-CAAX

    Plasmid
    #75176
    Purpose
    Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTagBFP-CAAX
    Use
    Three prime entry vector for multisite gateway th…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75176-plasmid-map-sequence-id-154502

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  17. MSP2253

    Plasmid
    #70704
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70704-plasmid-map-sequence-id-127764

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  18. pT7-agrBD-I

    Plasmid
    #53439
    Purpose
    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220
    Depositor
    Cynthia Collins
    Publication
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7-lac
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53439-plasmid-map-sequence-id-83233

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Showing: 5261 - 5280 of 18247 results