We narrowed to 6,480 results for: poly

1. pCR8/GW/TOPO BMI

   Plasmid

   #69796

   Purpose

   Gateway entry vector for BMI

   Depositor

   Gerard Cagney

   Insert

   BMI (BMI1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

2. 5TEY (METTL14)

   Plasmid

   #101891

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   METTL14 (METTL14 Human)
   

   Use

   Baculovirus expression

   Tags

   Expression

   Mutation

   Promoter

   polyhedrin

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pCAGGS-NICD

   Plasmid

   #26891

   Depositor

   Nicholas Gaiano

   Article

   Dang et al Dev Neurosci. 2006;28(1-2):58-69.

   Insert

   NICD (Notch1 Mouse)
   

   Use

   Tags

   FLAG and b-globin polyA

   Expression

   Mammalian

   Mutation

   E2299K

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

4. Tgfb-Induced-Enhancer:Beta-globin-minimal-promoter-EGFP:pA pDestTol2pA2

   Plasmid

   #220508

   Purpose

   TGFb-inducible fluorescent reporter (TIE:EGFP)

   Depositor

   Leonard Zon

   Inserts

   TGFb inducible enhancer
   beta-globin minimal promoter
   SV40 PolyA signal
   

   Use

   Zebrafish expression (tol2)

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   June 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p404TDH3

   Plasmid

   #15973

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   TDH3 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LEGO-AP1x3-GM55

   Plasmid

   #217414

   Purpose

   Luciferase expression vector with 3 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x3

   Depositor

   Peter Cockerill

   Insert

   Luciferase, GFP
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   3 AP-1 sites, Human -55 to +28 minimal CSF2 promo…

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 5E26 (PANK2)

   Plasmid

   #79516

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PANK2 (PANK2 Human)
   

   Use

   Baculovirus expression

   Tags

   6xHis and TEV cleavage site

   Expression

   Mutation

   Promoter

   polyhedrin

   Available since

   June 15, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pIK86

   Plasmid

   #47933

   Purpose

   C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.

   Depositor

   Iskra Katic

   Article

   Katic et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR and Unspecified

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. TYK2A-c075

   Plasmid

   #53688

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TYK2A (TYK2 Human)
   

   Use

   Baculovirus expression

   Tags

   His6-TEV

   Expression

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. p405MET25

    Plasmid

    #15961

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MET25 promoter is between SacI and XbaI restricti…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 4QPP

    Plasmid

    #61964

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HRMT1L6:PBC001-A12:C213642 (PRMT6 Human)
    

    Use

    Baculovirus expression

    Tags

    6x His and TEV cleavage site

    Expression

    Mutation

    See comments

    Promoter

    Polyhedrin

    Available since

    Sept. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pFastBac1-Nucleocapsid

    Plasmid

    #179696

    Purpose

    Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells

    Depositor

    Mark Arbing

    Insert

    SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    His6-TEV protease site

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. WDR61

    Plasmid

    #28162

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WD repeat domain 61 (SKIC8 Human)
    

    Use

    Baculovirus expression

    Tags

    His tag with TEV cleavage

    Expression

    Mutation

    Promoter

    polyhedrin

    Available since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. BDH2_FL_BioN

    Plasmid

    #195870

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    BDH2 (BDH2 Human)
    

    Use

    Pfbd-bira, baculovirus expression

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRAV12

    Plasmid

    #67713

    Purpose

    Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification

    Depositor

    Jeffrey Kieft, Robert Batey

    Article

    Kieft et al RNA. 2004 Jun;10(6):988-95.

    Insert

    Transcription cassette for 3'-tagged RNAs
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA polymerase

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p2Bac.V5.p62

    Plasmid

    #39218

    Depositor

    Aziz Sancar

    Insert

    p62 (GTF2H1 Human)
    

    Use

    Tags

    V5

    Expression

    Insect

    Mutation

    Promoter

    Pp10

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

17. ACSM2B

    Plasmid

    #39167

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ACSM2B (ACSM2B Human)
    

    Use

    Baculovirus expression

    Tags

    His Tev

    Expression

    Mutation

    Actually the mitochondrial form, GI:58082049, wit…

    Promoter

    polyhedrin

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pUPD2:35S-TMV-Rep-MP

    Plasmid

    #106217

    Purpose

    Provides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)

    Depositor

    Tomáš Moravec

    Insert

    Infectious clone of Tobacco mosaic virus, 5' part
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2+-dCas9-olEzh2

    Plasmid

    #180216

    Purpose

    Ezh2 fused to dCas9 for H3K27me3 epigenome editing

    Depositor

    Hiroyuki Takeda

    Article

    Fukushima et al Epigenetics Chromatin. 2019 Mar 13;12(1)

    Insert

    enhancer of zeste 2 polycomb repressive complex 2 subunit
    

    Use

    Tags

    FLAG and dCas9

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pfbiRS

    Plasmid

    #67283

    Purpose

    Expresses the fbiC gene from Mycobacterium smegmatis

    Depositor

    Aziz Sancar

    Article

    Selby et al Biochemistry. 2012 Jan 10;51(1):167-71.

    Insert

    fbiC gene from Mycobacterium smegmatis
    

    Use

    Tags

    polyhistidine

    Expression

    Mammalian

    Mutation

    non known

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only