We narrowed to 13,571 results for: GAN

1. pLenti-multi-CRISPR-sgRipk3_#2-puro

   Plasmid

   #231982

   Purpose

   Knockout mouse Ripk3

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-multi-CRISPR-sgRipk3_#1-puro

   Plasmid

   #231981

   Purpose

   Knockout mouse Ripk3

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-multi-CRISPR-sgMlkl_#2-puro

   Plasmid

   #231980

   Purpose

   Knockout mouse Mlkl

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Mlkl Mouse)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-multi-CRISPR-sgMlkl_#1-puro

   Plasmid

   #231979

   Purpose

   Knockout mouse Mlkl

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Mlkl Mouse)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX MLKL(1-180)-2A-mCherry-puro

   Plasmid

   #231976

   Purpose

   Tet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosis

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   MLKL 1-180 construct with bi-cistronic mCherry (Mlkl Mouse)
   

   Use

   Lentiviral

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX MLKL(S345D)-2A-mCherry-puro

   Plasmid

   #231975

   Purpose

   Tet inducible expression of DmrB-Mlkl with mCherry to induce phosphomimetic MLKL S345D necroptosis

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   MLKL S345D construct with bi-cistronic mCherry (Mlkl Mouse)
   

   Use

   Lentiviral

   Mutation

   MLKL S345D

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. 8R6A

   Plasmid

   #228446

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Article

   Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

   Insert

   DTX1 (DTX1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. hTln1-R12DD-SCLS

   Plasmid

   #191443

   Purpose

   expresses a variant of human talin1 domains R12-DD, with a deletion between K2375 and A2397

   Depositor

   Ben Goult

   Article

   Elefant et al JCI Insight. 2024 Dec 20;9(24):e173664.

   Insert

   Human Talin1 R12DD (TLN1 Human)
   

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   deletion between residues K2375 and A2397

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF_his6_sumo_ZIKV_NS5_MTase

   Plasmid

   #213529

   Purpose

   Expresses the MTase domain of NS5 of ZIKV

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   ZIKA virus NS5 methyltransferase domain
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   MTase domain aa 1-265

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX304-BCL-XL-F97W

    Plasmid

    #203572

    Purpose

    Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

    Depositor

    Abhishek Chakraborty

    Article

    Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5-tagged

    Expression

    Mammalian

    Mutation

    Changed Phenylalanine 97 to Tryptophan for partia…

    Promoter

    CMV

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBbsr2-EREG-ScNeo

    Plasmid

    #209909

    Purpose

    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EREG-ScNeo (EREG Human)
    

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPBpuro-miRFP703-eDHFR(69K6)-cRaf

    Plasmid

    #209919

    Purpose

    Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    miRFP703-eDHFR(69K6)-cRaf (RAF1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPBbleo-miRFP703-eDHFR(69K6)-cRaf

    Plasmid

    #209920

    Purpose

    Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    miRFP703-eDHFR(69K6)-cRaf (RAF1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPBbsr2-miRFP703-eDHFR(69K6)-cRaf

    Plasmid

    #209921

    Purpose

    Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    miRFP703-eDHFR(69K6)-cRaf (RAF1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPBbleo-HBEGF-ScNeo

    Plasmid

    #209903

    Purpose

    To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    HBEGF-ScNeo (HBEGF Human)
    

    Tags

    mNeonGreen and mScarlet

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBbsr2-HBEGF-ScNeo

    Plasmid

    #209907

    Purpose

    To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    HBEGF-ScNeo (HBEGF Human)
    

    Tags

    mNeonGreen and mScarlet

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKM-U6-reRNA-mmDNMT1

    Plasmid

    #205635

    Purpose

    reRNA guide targeting Mus Musculus DNMT1

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    reRNA-mmDNMT1 (Dnmt1 Mouse)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDual

    Plasmid

    #207883

    Purpose

    Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.

    Depositor

    David Edgell

    Article

    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

    Inserts

    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Leucine 212 changed to proline, leucine 434 in th…

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Beta1 Q280* K418* ectodomain

    Plasmid

    #221399

    Purpose

    Mammalian expression of human integrin beta1 Q280* K418* ectodomain

    Depositor

    Timothy Springer

    Article

    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

    Insert

    integrin beta1 Q280* K418* ectodomain (ITGB1 Human)
    

    Tags

    AVI tag, HRV3C cleavage site, basic coil, HA tag,…

    Expression

    Mammalian

    Mutation

    codon optimized for human mature _1 residues Q1 t…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Beta1 N391* N562* ectodomain

    Plasmid

    #221400

    Purpose

    Mammalian expression of human integrin beta1 N391* N562* ectodomain

    Depositor

    Timothy Springer

    Article

    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

    Insert

    integrin beta1 N391* N562* ectodomain (ITGB1 Human)
    

    Tags

    AVI tag, HRV3C cleavage site, basic coil, HA tag,…

    Expression

    Mammalian

    Mutation

    codon optimized for human mature _1 residues Q1 t…

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLX304-BCL-XL-499R

    Plasmid

    #203574

    Purpose

    Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499) in mammalian cells.

    Depositor

    Abhishek Chakraborty

    Article

    Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5- tagged

    Expression

    Mammalian

    Mutation

    silent point mutations introduced to make it resi…

    Promoter

    CMV

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. Lenti-sgRNA-Cre-GpNLuc-sgmMLKL

    Plasmid

    #208391

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MLKL gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgMLKL (Mlkl Mouse)
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PABPC1_544-636_pGEX6P1

    Plasmid

    #209789

    Purpose

    Bacterial expression of PABPC1 Mlle domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al EMBO J. 2004 Jan 28;23(2):272-81. doi: 1

    Insert

    Mlle domain of PABPC1 (PABPC1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 544-636 only

    Promoter

    tac

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAG426GAL-FES

    Plasmid

    #203501

    Purpose

    Expresses human FES kinase from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    FES (FES Human)
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAG416GAL10-c-Src

    Plasmid

    #203462

    Purpose

    Expresses human c-Src kinase from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    c-Src (CSK Human)
    

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAG416GALL-Bat-CoV-HKU9_3CL

    Plasmid

    #203478

    Purpose

    Expresses Bat-CoV HKU9 3CL protease from a GALL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    Bat-CoV HKU9 3CL protease (ORF1ab )
    

    Expression

    Yeast

    Promoter

    GALL

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A_S

    Plasmid

    #147515

    Purpose

    Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A (gw Nematode, Human)
    

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A_R

    Plasmid

    #147389

    Purpose

    Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A (gw Nematode, Fly)
    

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_635-861_R

    Plasmid

    #147404

    Purpose

    Insect Expression of CeAIN2_254-400-DmGW182_635-861

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-DmGW182_635-861 (gw Nematode, Fly)
    

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_635-1384_R

    Plasmid

    #147405

    Purpose

    Insect Expression of CeAIN2_254-400-DmGW182_635-1384

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-DmGW182_635-1384 (gw Nematode, Fly)
    

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV_GFP11-14-SEPT7

    Plasmid

    #180342

    Purpose

    mammalian expression of human SEPT7 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN7 (SEPTIN7 Human)
    

    Tags

    GFP11

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV_SEPT9_i1-14-GFP11

    Plasmid

    #180344

    Purpose

    mammalian expression of human SEPT9_i1 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    GFP11

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV_SEPT9_i3-14-GFP10

    Plasmid

    #180345

    Purpose

    mammalian expression of human SEPT9_i3 fused to GFP10

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN9_v3 (SEPTIN9 Human)
    

    Tags

    GFP10

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV_SEPT9_i3-14-GFP11

    Plasmid

    #180346

    Purpose

    mammalian expression of human SEPT9_i3 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN9_v3 (SEPTIN9 Human)
    

    Tags

    GFP11

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCEP4 TAPBPR-TM-TN6

    Plasmid

    #178650

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    TAPBPR (TAPBPL Human)
    

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    E205K, R207E, Q209S, Q272S, switched transmembran…

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCS2plus-TDP-43

    Plasmid

    #162608

    Purpose

    Allows for transcription of TDP-43 mRNA for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    TARDBP (TARDBP Human)
    

    Expression

    Mammalian

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGG442

    Plasmid

    #165616

    Purpose

    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    

    Use

    CRISPR

    Tags

    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

    Expression

    Yeast

    Mutation

    Corrected homology relative to wild type SpCas9 (…

    Promoter

    TEF1

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Myc-KLC1 delta Tail

    Plasmid

    #166965

    Purpose

    Expresses Myc-tagged KLC1 protein (1-495 aa) in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KLC1 (Klc1 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    KLC1 (1-495aa)

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FLAG-KIF5AR280H

    Plasmid

    #166957

    Purpose

    Expresses FLAG-tagged KIF5A R280H protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A-R280H (Kif5a Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    KIF5A-R280H

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV ShadowG-AURKA K162M-mTurq2

    Plasmid

    #157769

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and shadowG

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge