We narrowed to 7,181 results for: RAP

1. ABE8.8-m

   Plasmid

   #136294

   Purpose

   expresses ABE8.8-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.8-m
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mito-mGreenLantern

   Plasmid

   #164461

   Purpose

   Localization of mGreenLantern fluorescent protein to mitochondria

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   Human COX8a first 29 amino acids

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

   Plasmid

   #188385

   Purpose

   Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTP261

   Plasmid

   #104166

   Purpose

   Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

   Depositor

   Dirk Görlich

   Article

   Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

   Insert

   Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
   

   Use

   Tags

   14x Histidine tag and NEDD8 from Brachypodium dis…

   Expression

   Bacterial

   Mutation

   1x Cysteine located at bps 493-495 in full sequen…

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBpaRS3tRNA

   Plasmid

   #197102

   Purpose

   To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

   Insert

   TyrRS variant, amber suppressor tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMP71-tCD19-mGFP

   Plasmid

   #174609

   Purpose

   expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   CD19 extracellular and transmembrane domain (Cd19 Mouse)
   

   Use

   Tags

   palmitoylated EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Luc-T20

   Plasmid

   #177941

   Purpose

   Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   Luc2 (ORF1ab )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRIPZ shcGAS-Mmu

   Plasmid

   #127698

   Purpose

   Doxycyclin inducible shRNA knockdown of mouse cGAS gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shcGAS Mmu (Cgas Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tubulin-mGreenLantern

   Plasmid

   #164460

   Purpose

   Localization of mGreenLantern fluorescent protein to tubulin

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   Tubulin

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

    Plasmid

    #188383

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-fDIO-pAce-Kv2.1PR

    Plasmid

    #195535

    Purpose

    Flp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAce-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Ace-mNeon 78K, 81D, 92N, 178F; SY linker

    Promoter

    CAG

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Luc-PS9

    Plasmid

    #177942

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2 (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2

    Plasmid

    #170823

    Purpose

    PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d PPP2CA gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR221-JUN

    Plasmid

    #79512

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    JUN (JUN Human)
    

    Use

    Multisite gateway entry clone

    Tags

    HA Tag

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-EF1A-mSTING

    Plasmid

    #229739

    Purpose

    Expresses mouse STING in mammalian cells. Stable expression with piggybac transposase.

    Depositor

    Shawn Ferguson

    Article

    Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

    Insert

    STING (Sting1 Mouse)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

    Plasmid

    #126580

    Purpose

    A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. H2B-mGreenLantern

    Plasmid

    #164464

    Purpose

    Localization of mGreenLantern fluorescent protein to histone 2B

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Human Histone 2B (D26G and V119I mutations)

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PL752-EN1-LA-p2a-mTagBFP2nls-3xHAtag-hGHpA-PGK-puroR-EN1-RA

    Plasmid

    #191691

    Purpose

    For knocking p2a-mTagBFP2nls-3xHAtag into EN1 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    EN1 homology arms and p2a-mTagBFP2nls-3xHAtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-HA-GST-4EBP1-Low GC

    Plasmid

    #69018

    Purpose

    expresses tagged 4EBP1

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    EIF4EBP1 (EIF4EBP1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    lowGC content, wt is ~70% GC rich. Still, it code…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRIPZ shIFNAR

    Plasmid

    #127699

    Purpose

    Doxycyclin inducible shRNA knockdown of mouse IFNAR gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shIFNAR1 Mmu (Ifnar1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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