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We narrowed to 25,138 results for: crispr

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Showing: 5281 - 5300 of 25138 results

  3. sgRNA1_Tet-inducible Luciferase reporter

    Plasmid
    #64161
    Purpose
    Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.
    Depositor
    Moritoshi Sato
    Publication
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA1 for Tet-inducible Luciferase reporter
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    human U6
    Available since
    June 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64161-plasmid-map-sequence-id-116856

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  5. pS548∙CsR

    Plasmid
    #197859
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Publication
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold - Tc resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197859-plasmid-map-sequence-id-393256

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  6. pXGFPC-5 Pxyl-dcas9 (Sth3)

    Plasmid
    #133317
    Purpose
    expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dcas9 (Streptococcus thermophilus #3)
    Use
    Unspecified
    Promoter
    xylose
    Available since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133317-plasmid-map-sequence-id-266835

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  7. pSBY3_ligD

    Plasmid
    #104624
    Purpose
    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD
    Depositor
    Sheng Yang
    Publication
    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    FnCpf1 codon-optimized C.glutamicum
    Promoter
    hsp60
    Available since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104624-plasmid-map-sequence-id-201663

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  9. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid
    #74709
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA
    Depositor
    Eugene Yeo
    Publication
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. PX458_ZNF219_1

    Plasmid
    #72368
    Purpose
    Encodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZNF219 (ZNF219 Human)
    Use
    CRISPR
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72368-plasmid-map-sequence-id-132118

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  14. pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

    Plasmid
    #187952
    Purpose
    FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-CasRx-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker, HA-tag, and NLS
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187952-plasmid-map-sequence-id-373488

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  15. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid
    #140000
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140000-plasmid-map-sequence-id-275479

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  20. pAK_EF1a_CasRx_Hygro

    Plasmid
    #134840
    Purpose
    Lentiviral expression of CasRx T2A Hygromycin
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Hygromycin
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134840-plasmid-map-sequence-id-266452

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Showing: 5281 - 5300 of 25138 results