170,819 results

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pFA6a- mEGFP-kanMX6

Plasmid

#105146

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mEGFP-kanMX6

Tags

mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_TNIK

Plasmid

#45276

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TNIK (TNIK Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pPolI_SARS-CoV-2_NLuc

Plasmid

#183953

Purpose

Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

Depositor

AJ te Velthuis

Article

Vial et al bioRxiv 2021.06.28.450211

Insert

SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)

Expression

Mammalian

Promoter

Mammalian Pol I

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-L

Plasmid

#182279

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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CROPseq-tRFP

Plasmid

#230938

Purpose

Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

Depositor

Hyejung Won

Article

Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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CLYBL_Bxb1-GA_LP_Dual_TC

Plasmid

#229776

Purpose

CLYBL targeting construct with the Bxb1-GA landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

FRT-mScarlet-attP(GA)-PuroR-F3

Use

Synthetic Biology

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLX-TRE-dCas9-KRAB-MeCP2-BSD

Plasmid

#140690

Purpose

Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

Cas9m4-KRAB-MeCP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003 S49C

Plasmid

#133448

Purpose

Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003 S49C

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Contains S49C mutation

Promoter

T7

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-OTI-TCRA_p2_TCRB

Plasmid

#131033

Purpose

Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Inserts

TCRA (Tcra Mouse)
TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV/T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-hATG2A

Plasmid

#114462

Purpose

Expresses human ATG2A with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

Insert

human ATG2A (ATG2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

P656R (please see depositors comment below)

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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