Skip to main content
Addgene
Showing: 10601 - 10640 of 23940 results

  2. pdCas9

    Plasmid
    #46569
    Purpose
    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46569-plasmid-map-sequence-id-364492

    Enlarge

  6. pTP1172

    Plasmid
    #104161
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104161-plasmid-map-sequence-id-200072

    Enlarge

  10. pCEP4-SERT-myc

    Plasmid
    #107456
    Purpose
    Mammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107456-plasmid-map-sequence-id-205545

    Enlarge

  12. SETDB1

    Plasmid
    #25240
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETDB1, SETDB1_16 (SETDB1 Human)
    Use
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pSico

    Plasmid
    #11578
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11578-plasmid-map-sequence-id-106042

    Enlarge

  23. pYC-C

    Plasmid
    #207114
    Purpose
    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus
    Depositor
    Yifan Cheng
    Article
    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207114-plasmid-map-sequence-id-414780

    Enlarge

  27. pVS2

    Plasmid
    #33144
    Depositor
    Michael Rosbash
    Article
    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.
    Inserts
    LacO (256 copies)
    GAL-GFP-kd200-GALpA reporter
    Use
    Tags
    Expression
    Yeast
    Mutation
    GFP is mutated such that amino acids 64-73 (FSYGV…
    Promoter
    bidirectional GAL1-GAL10 promoter
    Available since
    Dec. 20, 2011
    Availability
    Academic Institutions and Nonprofits only

  29. MSGV Mouse Acceptor

    Plasmid
    #128176
    Purpose
    A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9
    Depositor
    David Ott
    Article
    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    MSCV
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128176-plasmid-map-sequence-id-252503

    Enlarge

  32. Lenti-UbC-LARGE1-EF1a-BSD

    Plasmid
    #205151
    Purpose
    Express UbC-driven LARGE1 and EF-1a driven BSD
    Depositor
    Monkol Lek
    Insert
    LARGE xylosyl- and glucuronyltransferase 1 (LARGE1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    205151-plasmid-map-sequence-id-405996

    Enlarge

  36. cytoplasmic NAD+ biosensor

    Plasmid
    #186787
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm
    Depositor
    Michael Cohen
    Article
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    cytoplasmic NAD+ biosensor
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186787-plasmid-map-sequence-id-368056

    Enlarge

  38. Mouse 3' Hp1a AID GFP PuroR

    Plasmid
    #127897
    Purpose
    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene
    Depositor
    Mark Groudine
    Insert
    Hp1a (Cbx5 Mustard Weed, Mouse)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Expression
    Mutation
    Inserting GFP AID 2A Puro into mouse 3' Hp1a
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127897-plasmid-map-sequence-id-250990

    Enlarge

  39. pYC-N

    Plasmid
    #207113
    Purpose
    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus
    Depositor
    Yifan Cheng
    Article
    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    207113-plasmid-map-sequence-id-410641

    Enlarge

Showing: 10601 - 10640 of 23940 results