We narrowed to 11,805 results for: NSI

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pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag

Plasmid

#191537

Purpose

Overexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-ddMisCas13b-SNAPf-NLS-3xFlag

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Flag Snail WT

Plasmid

#16218

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo1-G3BP1_pTRE-G418

Plasmid

#202015

Purpose

expresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-LOV-Turbo1-G3BP1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

pTRE-Tight

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

Plasmid

#197687

Purpose

plasmid for expressing the DNMT3bCD

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET-16b-10His-TEV-MCES_VACCA_D1_D12

Plasmid

#203671

Purpose

The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

Depositor

Stephen Cusack

Article

Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

Insert

MCES_VACCA_D1_D12

Tags

10xHis

Expression

Bacterial

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-PD-L1-SNAP

Plasmid

#223577

Purpose

Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-CKAR(T/A)

Plasmid

#118410

Purpose

Negative-control mutant for ExRai-CKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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∆DUSP6

Plasmid

#27977

Depositor

Igor Astsaturov

Article

Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

Insert

DUSP6 (DUSP6 Human)

Use

Lentiviral

Tags

HA and MYC

Expression

Mammalian

Mutation

C293S: mutation in the phosphatase domain

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
GFP-PKCA D463N

Plasmid

#195203

Purpose

Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

Depositor

David Bryant, Peter Parker

Article

Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

Insert

PRKCA (PRKCA Human)

Expression

Mammalian

Mutation

D463N

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-CAG-DEST

Plasmid

#80489

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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Myristoylated optoDroplet

Plasmid

#111507

Purpose

For membrane localized optoDroplets

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

Cry2 (CRY2 Mustard Weed)

Use

Lentiviral

Tags

FUS, Fusion Red, and Myristoylation Sequence (MYR…

Promoter

SFFV

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMTMVi-N

Plasmid

#118756

Purpose

Intermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant.

Depositor

Jose-Antonio Daros

Article

Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

Insert

Tobacco mosaic virus cDNA fragment with a cloning polylinker

Use

Intermediate to build tobacco mosaic virus recomb…

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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GFP Snail WT

Plasmid

#16225

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pmEGFP-C1-Nir3-LNS2

Plasmid

#220087

Purpose

C-terminal PA binding domain of Nir3

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM2 (PITPNM2 Human)

Tags

mEGFP-GGGGSHM

Expression

Mammalian

Mutation

amino acids 925-1209

Promoter

CMV

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc rat SAPAP1

Plasmid

#40215

Depositor

Yutaka Hata, Yoshimi Takai

Article

Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

Insert

SAPAP1 (Dlgap1 Rat)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

Plasmid

#191159

Purpose

Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mTagBFP:EGFP.s2:mCherry

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-KRaION1

Plasmid

#177799

Purpose

Bacterial expression of green fluorescent potassium indicator KRaION1

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

KRaION1

Expression

Bacterial

Promoter

araB

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_1G11F2-3 light chain

Plasmid

#198254

Purpose

recombinant expression of light chain of monoclonal anti-AMP antibody 1G11F2-3

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

light chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse kappa)

Tags

IL10 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Plasmid

#177919

Purpose

Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PA-B/BRISBANE/60/2008

Plasmid

#187920

Purpose

Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase acidic protein

Tags

UNTAGGED

Expression

Mammalian

Promoter

CMV Promoter

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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