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pCAG-MBP-ULK1-1-290-mcherrry

Plasmid

#249732

Purpose

Expressing human ULK1-1-290 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

1-290

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ULK1-290-835-mcherry

Plasmid

#249733

Purpose

Expressing human ULK1-290-835 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

290-835

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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p1.2-GS

Plasmid

#162770

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

Type

Empty backbone

Expression

Mammalian

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.2-ABE8e

Plasmid

#185669

Purpose

Expresses eNme2-T.2-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-ABCB1_STOP

Plasmid

#221424

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

Insert

ABCB1 (ABCB1 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pEND-351_Cacnes_dCas9_CRISPRi

Plasmid

#225617

Purpose

Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

dCas9

Use

CRISPR and Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-ABE7.10-NG

Plasmid

#181753

Purpose

MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE7.10-NG

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS2114

Plasmid

#49139

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-emGFP

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG_mCherry-ATG13 (WT)

Plasmid

#223735

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 (ATG13 Human)

Tags

mCherry

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pET-TRSter

Plasmid

#78865

Purpose

Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

Depositor

Elias Arnér

Article

Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

Insert

Thioredoxin Reductase (Txnrd1 Rat)

Tags

Mutated E. coli-type SECIS element (3’ end of ins…

Expression

Bacterial

Promoter

T7lac

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-APEX2-SOPP3-3xNLS

Plasmid

#229074

Purpose

expresses APEX2-SOPP3 in the mammalian nucleus

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the mammalian nucleus (APEX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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CRY2-mCherry-Miro1TM

Plasmid

#102247

Purpose

Expresses fusion of CRY2PHR with mCherry and transmembrane domain of Miro1

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

CRY2PHR-mCherry-Miro1TM (RHOT1, CRY2 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

Plasmid

#176027

Purpose

construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

Depositor

Irmela Jeremias

Article

Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

Inserts

rtTA3
Luc2
mtagBFP

Use

Lentiviral

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCEFL_GPR161-HA

Plasmid

#249268

Purpose

Expression of GPR161 wild type

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GPR161 (GPR161 Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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Orf3a-mCherry

Plasmid

#165138

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Orf3a (ORF3a codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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hCathepsin S

Plasmid

#11251

Purpose

Mammalian expression of human Cathepsin S

Depositor

Hyeryun Choe

Article

Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

Insert

cathepsin S (CTSS Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pC1-IMS-oROS-HT

Plasmid

#216419

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the inter-membrane-space of mitochondria.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-USP7

Plasmid

#99393

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

USP7 (USP7 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Nsp3 -mCherry

Plasmid

#165131

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp3 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadCBEd-V106W-BlastR

Plasmid

#193849

Purpose

Express TadCBEd-V106W in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd-V106W

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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