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pmKalama1-Nuc

Plasmid

#14894

Purpose

Mammalian expression of mKalama1 blue fluorescent protein with NLS

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

pmKalama1-Nuc

Tags

NLS

Expression

Mammalian

Mutation

I178T

Promoter

CMV

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-hsyn-ASAP5-WPRE

Plasmid

#225709

Purpose

Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5

Use

AAV

Promoter

human synapsin

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-RhoA-wt

Plasmid

#12959

Depositor

Gary Bokoch

Insert

RhoA (RHOA Human)

Tags

GST

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pSBbi G418R mbIL21

Plasmid

#194329

Purpose

Consitiutive mammalian expression of membrane bound human IL-21

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

mbIL-21 (IL21 Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TDsmURFP-IRES-eGFP

Plasmid

#80344

Purpose

Expresses Tandem Dimer smURFP and eGFP in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
eGFP

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAC-ZEAXipi

Plasmid

#53287

Purpose

Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, idi, crtY, crtI, crtB, crtZ

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRK6-HA-TAK1

Plasmid

#44160

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-TGF beta Type II receptor Flag

Plasmid

#31719

Depositor

Rik Derynck

Insert

TGF beta Type II Receptor (TGFBR2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only
PKC beta II DN

Plasmid

#16385

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97(wt)

Plasmid

#14666

Depositor

Graham Warren

Article

Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Insert

p97 (Vcp Mouse)

Tags

RGS6xHis

Expression

Bacterial

Mutation

See depositor comments below.

Available Since

April 13, 2007

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast

Plasmid

#125133

Purpose

Lentiviral empty backbone control vector with CMV promoter and Blasticidin resistance gene

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Type

Empty backbone

Use

Lentiviral

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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HO-Poly-KanMX4-HO

Plasmid

#51662

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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PL-452 C-EGFP

Plasmid

#19178

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

EGFP

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-HA-hIFITM2

Plasmid

#58398

Purpose

Expressed human IFITM2 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM2 (IFITM2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pAD48-daf-2 RNAi

Plasmid

#34834

Depositor

Cynthia Kenyon

Article

Dillin et al Science. 2002 Oct 25;298(5594):830-4.

Insert

daf-2 (daf-2 Nematode)

Use

RNAi

Expression

Bacterial

Available Since

June 11, 2012

Availability

Academic Institutions and Nonprofits only
pBS-hBAF60a

Plasmid

#21034

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF60a (SMARCD1 Human)

Use

Phagemid

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pKeima-LiveDrop

Plasmid

#199695

Purpose

lipophagy reporter system based on the Keima-fluorophore

Depositor

Tobias Walther

Article

Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

Insert

GPAT4 (Gpat4 Fly)

Tags

Keima

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-NRF1

Plasmid

#34707

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pMT413

Plasmid

#8606

Depositor

Robert Hartley

Article

Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

Insert

barnase, barstar

Tags

phoA signal sequence to barnase

Expression

Bacterial

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
Opto-CRAC version 1

Plasmid

#101245

Purpose

Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

Depositor

Yubin Zhou

Article

He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

Insert

LOV2 and STIM1 cytosolic fragment

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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