We narrowed to 7,101 results for: tev

1. mTln1-F2F3-P229S-pET151

   Plasmid

   #177878

   Purpose

   Expresses mouse Talin 1 F2F3-P229S domains in bacterial cells

   Depositor

   Ben Goult

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   mouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   changed Proline 229 to Serine (P229S)

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-GST-ATG101-W110D/F112D

   Plasmid

   #188106

   Purpose

   Expresses human ATG101 HORMA W110D/F112D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG101 (ATG101 Human)
   

   Use

   Tags

   GST tag

   Expression

   Mammalian

   Mutation

   codon-optimized ATG101-W110D/F112D

   Promoter

   CMV

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX4T3-GST-PolB(K72A)

   Plasmid

   #177132

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG8H-GB1-EWS_171-264

   Plasmid

   #180466

   Purpose

   Expresses the construct His8-GB1-EWS_171-264 with a TEV cleavage site between GB1 and EWS_171-264

   Depositor

   David Libich

   Article

   Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   GB1 and His8

   Expression

   Bacterial

   Mutation

   deleted 1-170, 265-656

   Promoter

   T7

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG8H-GFP-EWS_1-120

   Plasmid

   #180464

   Purpose

   Expresses the construct His8-GFP-EWS_1-120 with a TEV cleavage site between GFP and EWS_1-120

   Depositor

   David Libich

   Article

   Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   GFP and His8

   Expression

   Bacterial

   Mutation

   deleted 121f-656

   Promoter

   T7

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJJGL003

   Plasmid

   #180607

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   DpbCasX with R3 loop
   

   Use

   CRISPR

   Tags

   10x His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX4T3-GST-NQO1(Y128F)

   Plasmid

   #177141

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of NQO1(Y128F) with a TEV protease site located between the GST tag and NQO1(Y128F)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   NQO1 (NQO1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. human TLNRD1-4H pET151

   Plasmid

   #159386

   Purpose

   Expresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cells

   Depositor

   Ben Goult

   Article

   Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   Talin Rod Domain Containing Protein 1 (TLNRD1 Human)
   

   Use

   Tags

   his-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   TLNRD1 4-helix domain (residues 143-273)

   Promoter

   T7

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDF11-SFPQ(214-598)_quadruple_mutant

   Plasmid

   #135441

   Purpose

   Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   splicing factor proline and glutamine rich (SFPQ Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   L535A, L539A, L546A, M549A

   Promoter

   T7

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pbIG-Xph20-Stg

    Plasmid

    #133035

    Purpose

    Expresses Xph20-Stg in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20-Stg
    

    Use

    Tags

    AviTag, His10, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pbIG-Xph18-ETWV

    Plasmid

    #133036

    Purpose

    Expresses Xph18-ETWV in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph18-ETWV
    

    Use

    Tags

    AviTag, His10, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pbIG-Xph18-Stg

    Plasmid

    #133037

    Purpose

    Expresses Xph18-Stg in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph18-Stg
    

    Use

    Tags

    AviTag, His10, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pbIG-Xph15-ETWV

    Plasmid

    #133038

    Purpose

    Expresses Xph15-ETWV in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph15-ETWV
    

    Use

    Tags

    AviTag, His10, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pbIG-Xph0-ETWV

    Plasmid

    #133039

    Purpose

    Expresses Xph0-ETWV in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph0-ETWV
    

    Use

    Tags

    AviTag, His10, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIG-TAT-Xph20-ETWV

    Plasmid

    #133040

    Purpose

    Expresses TAT-Xph20-ETWV in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20-ETWV
    

    Use

    Tags

    His8, TAT, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Plasmid

    #101210

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Plasmid

    #101203

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Plasmid

    #101212

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-22HT-MSI1(7-192)R53E/R61E

    Plasmid

    #60354

    Purpose

    R53E R61E double mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E and R61E mutations; contains amino acids 7-1…

    Promoter

    T7

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pEXP5-NT-5th R

    Plasmid

    #42572

    Depositor

    Ivan Korendovych

    Article

    Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

    Insert

    mutated calmodulin gene
    

    Use

    Tags

    Polyhistidine (6×His) region, TEV recognition sit…

    Expression

    Bacterial

    Mutation

    changed Phenylalanine 92 to Glutamine acid, Isole…

    Promoter

    T7

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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