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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,800 results for: emb

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Showing: 5321 - 5340 of 11800 results

  2. SNAP-CLC-EGFP

    Plasmid
    #193579
    Purpose
    Expresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells.
    Depositor
    Justin Taraska
    Publication
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Clathrin light chain B (CLTB Human)
    Tags
    SNAP-tag and mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193579-plasmid-map-sequence-id-380177

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  9. pAL725

    Plasmid
    #119862
    Purpose
    precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C
    Depositor
    Linda Randall
    Publication
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
    Tags
    none
    Expression
    Bacterial
    Mutation
    Leucine 244 to Cysteine, Aspartate 287 to Cysteine
    Promoter
    T5
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119862-plasmid-map-sequence-id-232032

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  10. DEP-GFP

    Plasmid
    #109043
    Purpose
    Expression of the Dishevelled DEP domain
    Depositor
    Mariann Bienz
    Publication
    Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dishevelled segment polarity protein 2 (DVL2 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Dvl2 DEP domain
    Promoter
    CMV
    Available since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    109043-plasmid-map-sequence-id-220610

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  11. ABCB8 (ABCB8A-c016)

    Plasmid
    #110266
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ABCB8 (ABCB8 Human)
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110266-plasmid-map-sequence-id-212276

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  14. pX330-sgRNA_Dicer_2

    Plasmid
    #68808
    Purpose
    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of
    Depositor
    Constance Ciaudo
    Publication
    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA mouse Dicer1
    Use
    CRISPR and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    68808-plasmid-map-sequence-id-120930

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  15. pcDNA 3.1 (+) Tacδ

    Plasmid
    #81177
    Purpose
    Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)
    Depositor
    Chinten James Lim
    Publication
    Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tacδ
    Tags
    KLGFFKR
    Expression
    Mammalian
    Mutation
    CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…
    Promoter
    CMV
    Available since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    81177-plasmid-map-sequence-id-169511

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  17. pChlr-1SE

    Plasmid
    #52140
    Purpose
    Encodes module 1 with amino acids 12S-16E for recognition of nt 8G
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 16Q to 16E in Module 1
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5321 - 5340 of 11800 results