We narrowed to 146,921 results for: ins;

1. pET28 acid.6

   Plasmid

   #191912

   Purpose

   Encodes a GFP design, acid.6

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   acid.6
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Q69L S72A Y145M T167V H181L

   Promoter

   T7

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28 sf:acid.3

   Plasmid

   #191926

   Purpose

   Encodes a GFP design, sf:acid.3

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:acid.3
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T65S Q69L S72A T108V Y145M V224I

   Promoter

   T7

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28 mid.7

   Plasmid

   #191900

   Purpose

   Encodes a GFP design, mid.7

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   mid.7
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   S72T Y145F V224I

   Promoter

   T7

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBABE puro mouse IRS-2

   Plasmid

   #11371

   Depositor

   Ronald Kahn

   Article

   Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

   Insert

   IRS-2 (Irs2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pBABE bleo human IRS-1

   Plasmid

   #11448

   Depositor

   Ronald Kahn

   Article

   Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

   Insert

   IRS-1 (IRS1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

6. MSP2279

   Plasmid

   #70706

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A and H557A in SaCas9

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pME-ReaChR-citrine

   Plasmid

   #169045

   Purpose

   Middle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert

   Depositor

   Paola Arlotta

   Article

   Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

   Insert

   ReaChR-citrine
   

   Use

   Gateway entry cloning vector

   Tags

   citrine

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX PLCg1(C)-SH2

   Plasmid

   #46469

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   PLCg1(C)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 656-766

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGen2.1-redCALWY-1

   Plasmid

   #50739

   Purpose

   Expresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.

   Depositor

   Maarten Merkx

   Article

   Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.

   Insert

   gene for redCALWY-1 Zn2+ sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX Syk(NC)-SH2

    Plasmid

    #46521

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Syk(NC) (SYK Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 7-263

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 3xFKBP-myc

    Plasmid

    #20213

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFKBP (FKBP1A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-GP-2-MPHOSPH8 Chromo

    Plasmid

    #59694

    Purpose

    Contains histone modification interacting domain (MPHOSPH8 Chromo)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    MPHOSPH8 Chromo (MPHOSPH8 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNP1 Aqua toehold switch

    Plasmid

    #107356

    Purpose

    Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 8 + Aquamarine
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. E. coli 16S toehold switch sensor

    Plasmid

    #110698

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    E. coli 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLTR PKC beta II

    Plasmid

    #8417

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Rat Protein Kinase C beta II (Prkcb Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

16. B. fragilis 16S toehold switch sensor

    Plasmid

    #110696

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    B. fragilis 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pExpreS2-1-C3-PA

    Plasmid

    #175446

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    3C-Protein A and BiP Secretion Peptide

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX Vav2-SH2

    Plasmid

    #46529

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Vav2 (VAV2 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 665-774

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a Gx-NL3

    Plasmid

    #169108

    Purpose

    Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX Syk(N)-SH2

    Plasmid

    #46520

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Syk(N) (SYK Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 7-114

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge