We narrowed to 17,934 results for: multi
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Plasmid#89368PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)DepositorInsertvhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)PromoterPosm-6Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDrVSP(C302S)-IRES2-EGFP
Plasmid#80331PurposeExpresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
ExpressionMammalianMutationC302SPromoterCMVAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVimentin-LSSmOrange
Plasmid#37134DepositorInsertLSSmOrange
Tagshuman cytochrome C oxidase subunit VIII and human…ExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-STU-Lb
Plasmid#138099PurposeGolden Gate entry vector for 2nd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM65879
Plasmid#153214PurposeLevel 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassetteDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HRE:GFP-pGK:Cre
Plasmid#68552PurposeLentivirus expressing hypoxia-responsive GFP reporter and CreDepositorInsertsGreen Fluorescent Protein
Cre
UseLentiviralExpressionMammalianPromoterHRE and pGKAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRel-Hyg
Plasmid#227582PurposeRelease plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 repliconDepositorInsertCas9
UseCRISPRAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_888
Plasmid#201165PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP-ura4MX6
Plasmid#33140Purposegenomic GFP tagging in yeastDepositorInsertsura4MX6
GFP
Available sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
PB-tetO-hNIL
Plasmid#197089PurposePiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiationDepositorAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBFC0619
Plasmid#186690PurposeVcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargoDepositorInsertstnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
ExpressionBacterialPromoterPLacAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pGFAP-AcGFP
Plasmid#133733PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a GFAP promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianPromoterpGFAP and pTRE3G-BiAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
LARG mCherry sspB in pcDNA3.1
Plasmid#90459PurposeRhoA mediated cell migrationDepositorInsertsExpressionMammalianMutationR73QPromoterCMVAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJA291
Plasmid#74487PurposeeF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFPDepositorInsertpuroR-mCherry-T2A-eGFP
UseLentiviralExpressionMammalianPromoterEF1alphaAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_EF1a-->GssmCherry-PNE
Plasmid#162157PurposeLentiviral vector for constitutive expression of mCherry and PNE anchor in mammalian cellsDepositorInsertmCherry
UseLentiviralExpressionMammalianAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-PDGFRA-reporter
Plasmid#136456PurposeMammalian fluorescent reporter plasmid for PDGFRA signaling.DepositorInsertsPDGFRalpha (PDGFRA Human)
Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianPromoterCMV and Minimal promoter with artificial SRE enha…Available sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BB3cK_pGAP_23*_pLAT1_Cas9
Plasmid#104908PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418DepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA
Plasmid#45789DepositorInsertsdeGFP
Tetracycline repressor
araC
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A
Plasmid#51248PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 849,865,877,907,980,1006 mutated to A…PromoterEF1aAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHelper_TS_V2_ampR
Plasmid#214467PurposeHelper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.DepositorPublicationInsertCspRecT / Bxb-1
UseSynthetic BiologyPromoterXylS / AraCAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only