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pCMV(MinDis)-iCyt_BrEt_SnFR_ER

Plasmid

#177746

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iCyt_BrEt_SnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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_pCMV(MinDis)-iCyt_F_SnFR_ER

Plasmid

#177744

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

_pCMV(MinDis)-iCyt_F_SnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iCytSnFR_ER

Plasmid

#177742

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iCytSnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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FMR1-E17B-P2A-NLuc

Plasmid

#157857

Purpose

donor plasmid for FMR1-Nluc

Depositor

Xinyu Zhao

Article

Li et al Stem Cells. 2017 Jan;35(1):158-169. doi:

Insert

FMR1-Nlu (FMR1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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EcNGsC-F307Y-pKK223

Plasmid

#131381

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307Y in Gs MutY.

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera F307Y

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are from…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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EcNGsC-F307A-pKK223

Plasmid

#131382

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307A in Gs MutY.

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera F307A

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are from…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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EcNGsC-S308V-pKK223

Plasmid

#131393

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308V in Gs MutY.

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera S308V

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are fro…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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EcNGsC-S308A-pKK223

Plasmid

#131394

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308A in Gs MutY.

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera S308A

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are fro…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA p21 K163R

Plasmid

#78787

Purpose

To overexpress p21 K163R in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Tags

HA

Expression

Mammalian

Mutation

K163R_mutation

Promoter

CMV

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA p21 K161R

Plasmid

#78788

Purpose

To overexpress p21 K161R in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Tags

HA

Expression

Mammalian

Mutation

K161R_mutation

Promoter

CMV

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG3_CH2charge

Plasmid

#105858

Purpose

Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG3 heavy chain (Ighg3 Mouse)

Expression

Mammalian

Mutation

mutated heavy chain of mouse IgG3, muatations: Hi…

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500

Plasmid

#92315

Purpose

Expression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

B1 domain of protein L (PL) mutant K16TAG

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70b (p70a promoter with one mutation)

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCS3-MT-BX-6MYC p21 1-89

Plasmid

#78784

Purpose

To overexpress p21 1-89 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Tags

6xMYC

Expression

Mammalian

Mutation

Jan-89

Promoter

CMV IE94

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
YF-AIP

Plasmid

#61525

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with EYFP and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP and EYFP

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
CF-AIP

Plasmid

#61526

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with ECFP and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP and ECFP

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-P3-Alb-mScarlet

Plasmid

#183461

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mScarlet

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-FLPo-Myc

Plasmid

#124641

Purpose

AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

Depositor

David Dupret

Article

Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

Insert

FLPo

Use

AAV and Cre/Lox

Tags

myc tag

Expression

Mammalian

Promoter

EF1a

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eGFP-VSV

Plasmid

#242781

Purpose

AAV transfer plasmid expressing eGFP-VSV under a CAG promoter.

Depositor

Andrew Payne

Article

Park et al bioRxiv 2025.09.26.678648

Insert

EGFP

Use

AAV

Tags

VSV G tag

Promoter

CAG

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-PalmGRET

Plasmid

#158221

Purpose

BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

PalmGFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCF159_BRL

Plasmid

#225962

Purpose

CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

CMV-Intron-BRL (env protein)

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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