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pOO2-GW

Plasmid

#135097

Purpose

Gateway vector for expression of proteins in Xenopus oocytes

Depositor

Wolf Frommer

Article

Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

Type

Empty backbone

Use

Bacterial expression vector for generating sp6 tr…

Promoter

SP6

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Myc-Bnip3FL

Plasmid

#100796

Purpose

Mammalian expression of Bnip3 full length splice variant

Depositor

Joseph Gordon

Article

Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

Insert

mus Bnip3 (Bnip3 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-ATP6V1D-HAGST

Plasmid

#87932

Purpose

C-term HA-GST tagged overexpression

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

ATP6V1D (ATP6V1D Human)

Tags

HA-GST

Expression

Mammalian

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 S166D S186D

Plasmid

#16236

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Mutation

Ser166 and Ser186 replaced by Asp

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pBS MCK promoter

Plasmid

#12528

Depositor

Ronald Kahn

Article

Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

Insert

MCK promoter (Ckm Mouse)

Expression

Mammalian

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-hNPPC-RFP

Plasmid

#22921

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human c-type natruiretic peptide precursor promoter driving DsRedExpress (NPPC Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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NET31 mRFP-N2 (490)

Plasmid

#61984

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al PLoS One. 2014 Nov 11;9(11):e111851. doi

Insert

NET31 (TMEM209 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-OTUB1 C91S-pcDNA3.1

Plasmid

#118210

Purpose

Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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TAK11

Plasmid

#227095

Purpose

Luciferase reporter vector with HIV-1 TAR region cloned in pGL3 Basic vector

Depositor

Farah Mustafa

Article

Khader et al Commun Biol. 2024 Nov 16;7(1):1521. doi:

Insert

CMV Promoter and HIV-1 TAR region

Use

Luciferase

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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TfR-pHoran4

Plasmid

#61547

Purpose

Human transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4

Depositor

David Perrais

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

Transferrin receptor (TFRC Human)

Tags

pHoran4

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuc-Nb37

Plasmid

#205515

Purpose

Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

nuc-Nb37

Tags

Nuclear localization signal (NLS) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcI-ts,ind+-(NSP8)

Plasmid

#160656

Purpose

Expresses codon optimized SARS-CoV-2 NSP8 in E. coli

Depositor

Kenneth Johnson

Article

Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

Insert

SARS-CoV-2 NSP8 (ORF1ab Synthetic)

Expression

Bacterial

Available Since

Dec. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a(+)-SUMO-A'

Plasmid

#209115

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_MYCL_P2A_Hygro_Barcode

Plasmid

#120462

Purpose

Barcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYCL (MYCL Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSR651

Plasmid

#65213

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-1 cap

Use

AAV

Expression

Insect

Promoter

p10 and polyhedrin

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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PM-FRB-CFP

Plasmid

#67517

Purpose

FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

mTOR (MTOR Human)

Tags

CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…

Expression

Mammalian

Mutation

FRB domain only (residues 2019–2114)

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pCD86-EGFP

Plasmid

#133858

Purpose

Mammalian expression of CD86 C-terminally tagged with EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

CD86 (CD86 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-CAG_3C-Twin-Strep

Plasmid

#113901

Purpose

Mammalian protein expression

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CAG

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)

Plasmid

#164041

Purpose

CRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B gene

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

mCherry-CRISPR-TagV2

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ABHD16A-Halo

Plasmid

#195163

Purpose

mammalian expression of siRNA-resistant ABHD16A tagged with Halo tag

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Tags

Halo tag

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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